PSMA基因沉默的前列腺癌细胞中肿瘤转移相关基因表达的芯片分析

Microarray analysis of tumor metastasis-related gene expression in PSMA-silencing prostate cancer cells

目的:探讨前列腺特异性膜抗原(prostate-specific membrane antigen,PSMA)在前列腺癌转移过程中的作用通路。方法:针对PSMA mRNA序列设计合成siRNA序列,脂质体转染LNCaP细胞特异性下调PSMA基因的表达,通过肿瘤转移基因芯片分析84个肿瘤转移相关基因的表达差异。结果:成功设计了siRNA序列并制备最佳干扰效果的干扰样,mRNA干扰效果达到75%以上,蛋白水平干扰效果达68%以上。通过基因芯片检测发现在下调PSMA基因表达后,有CDH6、CXCL12等10个基因发生了显著上调,而CCL7、MDM2等4个基因发生显著下调表达。结论:初步发现PSMA参与前列腺癌转移信号通路的调节,为进一步研究PSMA的生物学功能,探索前列腺癌的转移机制奠定了基础。

AIM： To investigate the functions of prostate-specific membrane antigen （PSMA） in prostate cancer metastasis. METHODS： Specific siRNA to knock down PSMA expression was designed and transfected into LNCaP cells. The tumor metastasis gene chip was also used to analyze the differential expression of 84 genes related to cancer metastasis. RESULTS： Specific siRNA was successfully designed and constructed and the gene expression of PSMA in LNCaP cells was knocked down. The RNAi efficiency was more than 75% at mRNA level and more than 68% at protein level. The results of the tumor metastasis gene chip indicated that 10 genes were up-regulated （such as CDH6 and CXCL12） and 4 genes were down-regulated （such as CCL7 and MDM2） in the LNCaP cells treated with PSMA siRNA. CONCLUSION： The PSMA is involved in the regulatory pathways in prostate cancer metastasis.