运动联合福善美对去卵巢大鼠骨密度和神经传导的影响

Effects of exercise combined with forsamax on bone mineral density and nerve conduction in ovariectomy-induced osteoporosis rats

目的:观察运动是否可增强福善美对卵巢切除大鼠骨质疏松的治疗作用。方法:将90只6月龄雌性SD大鼠随机分为假手术组(sham,18只)和卵巢切除模型组(OVX,72只)。大鼠卵巢切除8周后,测定大鼠第4腰椎骨密度(BMD)和血清雌二醇含量。随后,存活的OVX大鼠分为模型组(OVX)、福善美治疗组(OVX+FOX)、运动治疗组(OVX+EX)和福善美与运动联合治疗组(OVX+FOX+EX),分别给予1 mg.kg-1.d-1福善美灌胃和(或)跑台运动干预治疗12周后,双能X线骨密度仪测定各组大鼠第4腰椎BMD;肌电图机检测大鼠左侧股神经传导速度(MCV)、运动末端潜伏期(ML)和复合肌肉动作电位(CMAP);ELISA法测定大鼠血清Ⅰ型前胶原羧基端前肽(PICP)及Ⅰ型胶原羧基端交联端肽(ICTP)含量。结果:卵巢切除大鼠福善美和(或)运动干预治疗12周后,OVX组与sham组相比,BMD显著降低(P<0.05),血清PICP和ICTP明显增高(P<0.05),左侧股神经ML未见明显改变。福善美和运动均可显著提高骨质疏松大鼠BMD,降低ICTP;福善美可显著降低骨质疏松大鼠IC-TP,而运动对ICTP无明显影响。运动可明显缩短模型组左侧ML(P<0.05),福善美对ML无显著改善作用。运动与福善美联合对BMD、PICP、ICTP及ML的改善作用较两者单用效果显著(P<0.05);福善美与运动两治疗组间未见明显差异。各组大鼠左侧股神经MCV和CMAP未见明显差异。2×2析因设计的方差分析显示,福善美与运动2种处理方式之间不存在交互作用(P>0.05)。结论:福善美和运动可能通过抑制破骨细胞的骨吸收而抑制大鼠卵巢切除对骨密度的影响。

AIM： To observe the effects of exercise （EX） and forsamax （FOX） on bone mineral density （BMD） and nerve conduction in ovariectomized rats, and to determine whether exercise can enhance the effects of forsamax on postmenopausal osteoporosis （PMOP）. METHODS： Ninety 6-month-old female SD rats were randomly divided into 2 groups： sham group （18 rats） and ovariectomized group （72 rats）. Eight weeks after ovariectomy, dual-energy X-ray absorptiometry was applied to scan the BMD of the fourth lumbar vertebra. The level of serum estradiol （E2） was analyzed by ELISA. The living ovariectomized rats were then divided into 4 groups： ovariectomized model group （OVX）, fosamax treatment group （OVX＋FOX）, exercise treatment group （OVX＋EX）, and exercise combined with fosamax treatment group （OVX＋FOX＋EX）. After treated with fosamax （1 mg/kg intragastrically） and/or exercise for 12 weeks, the BMD of the fourth lumbar vertebra, the motor nerve conduction velocity （MCV）, motor distal latency （ML） and compound muscle action potential （CMAP） of the left femoral nerve were detected. The levels of serum type 1 procollagen carboxy-terminal propeptide （PICP） and type I collagen carboxy-terminal cross-linked telopeptide （ICTP） were also analyzed by ELISA. RESULTS： Twelve weeks after FOX and/or EX treatment, ovariectomized rats showed obviously lower BMD, higher PICP and ICTP than those in sham group （P〈0.05）. FOX or EX significantly increased BMD and reduced PICP induced by ovariectomy （P〈0.05）. FOX significantly reduced ICTP （P〈0.05）, but no remarkable difference was observed in OVX＋FOX group as compared with OVX group. EX＋FOX significantly increased BMD and reduced PICP and ICTP compared with FOX or EX alone （P〈0.05）. However, no obvious difference was observed between OVX＋FOX group and OVX＋EX group. No distinct difference in MCV and CMAP among the 5 groups was found. Neither ovariectomy nor FOX significantly affected ML. EX or EX＋FOX made ML shorter than that in OVX group （fP〈0.05）, and ML was remarkably shortened in OVX＋FOX＋EX group than that in FOX alone group on the left femoral nerve （P〈0.05）. No significant difference between FOX and EX in the protective effects against ovariectomized rats was observed. CONCLUSION： Exercise and fosamax may restrain bone absorption effect of osteoclast and then improve the bone mineral density in ovariectomized rats.