灵芝多糖抑制NADPH氧化酶表达防治大鼠动脉粥样硬化

The anti-atherosclerotic effect of Ganoderma lucidum Polysaccharides via down-regulation of vascular NADPH oxidases expression in atherosclerosis rats

目的探讨灵芝多糖对食饵性实验动脉粥样硬化(AS)大鼠的防治作用及其作用机制。方法将40只大鼠随机分为正常对照组、动脉粥样硬化模型组、灵芝多糖低、中、高剂量预防组。12周末颈动脉取血检测血清丙二醛(MDA)和超氧化物歧化酶(SOD)含量;处死大鼠,光镜观察各组主动脉粥样斑块形成情况;取主动脉测定活性氧(ROS)含量;免疫印迹观察其Nox4、p22phox蛋白表达情况。结果灵芝多糖各剂量组均可有效抑制动脉粥样硬化病理进程,降低血清MDA、SOD及主动脉ROS含量等氧化应激反应指标,减少主动脉Nox4、p22phox蛋白表达。结论灵芝多糖通过下调Nox4、p22phox等NADPH氧化酶蛋白表达水平抑制主动脉氧化应激反应,明显改善大鼠动脉粥样硬化。

Aim To study the molecular mechanism of preventive effect of Ganoderma lucidum Polysaccharides（GLP） on formation of atherosclerosis in the experimental atherosclerotic rats.Methods 40 rats were divided randomly into normal control group,atherosclerotic model group,treatment groups with GLP（125 mg·kg-1·d-1,250 mg·kg-1·d-1,500 mg·kg-1·d-1）.At the end of the 12th week after experiment,the concentration of serum SOD and MDA,the levels of aorta ROS,and the expressions of Nox4 and p22phox were determined respectively.And the rats were killed to obtain the formation of aorta arteriosclerosis with microscope.Results Compared with atherosclerotic model group,the levels of SOD,MDA,ROS of rats given GLP were lower.The formation of the aortic foam cells in the treatment group was lower.It may be related to the expressions of Nox4,p22phox proteins down-regulated by GLP.Conclusions The GLP has an anti-atherosclerotic effect in the experimental rats.The possible mechanism may be related to the regulation of NADPH oxidases.