CMTM2转基因小鼠的构建及其血清睾酮水平的变化

Establishment of a CMTM2 transgenic mouse model and the alteration of its serum testosterone level

目的:建立系统性表达CMTM2的转基因小鼠模型,研究CMTM2表达对小鼠生殖系统的影响。方法:通过显微注射pRevTRE-CMTM2表达载体建立CMTM2转基因小鼠;PCR鉴定CMTM2转基因小鼠基因型;RT-PCR、Western印迹、IHC方法检测CMTM2在转基因小鼠睾丸的表达情况;放免法检测转基因小鼠血清睾酮水平。结果:成功构建高表达CMTM2蛋白的转基因小鼠,并可以稳定传代;CMTM2转基因小鼠的血清睾酮水平较野生型小鼠提高[(46.04±3.72)nmol/L vs(42.43±3.80)nmol/L,P<0.05]。结论:成功构建CMTM2高表达的转基因小鼠,初步提示CMTM2基因可能对转基因小鼠睾酮的生成和分泌有一定影响。

Objective： To establish a transgenic mouse model systemically expressing the CMTM2 gene and study the effect of the CMTM2 expression on the reproductive system of mice in vivo. Methods： Transgenic mice were generated by microinjection of pRevTRE-CMTM2 and the genotype was detected by PCR. The expression of CMTM2 was determined by RT-PCR, Western blot and immunohistochemistry, and the serum testosterone level was measured by radioimmunoassay. Results： The CMTM2 gene was highly expressed in the testis of the transgenic mouse models and in their offspring as well. The level of serum testosterone was significantly in- creased in the transgenic models as compared with the wild-type mice （ [ 46.04 ±3.72 ] vs [ 42.43±3.80 ] nmol/L, P 〈 0.05 ）. Conclusion： The transgenic mouse model was established successfully, which could highly express the CMTM2 gene. It is indicated that CMTM2 may influence steroidogensis and testosterone secretion in transgenie mice.