侵袭性肺曲霉病小鼠肺组织差异表达蛋白的初步分析

Differential expression of proteins in the lung tissues of mice with invasive pulmonary Aspergillosis

目的初步分析侵袭性肺曲霉病(IPA)小鼠肺组织蛋白质的表达变化。方法运用双向电泳(2-DE)技术对正常组、正常感染组和IPA组小鼠肺组织总蛋白质进行分离,获得蛋白质表达谱;应用基质辅助激光解吸电离串联飞行时间质谱(MALDI-TOF/TOF)结合生物信息学进行蛋白质鉴定。结果初步鉴定出4个蛋白质点分别为过氧化物还原酶(peroxiredoxin-6,Prx6)、黄素还原酶(flavin reductase,FR)、Rho因子鸟苷酸解离抑制蛋白2(rho GDP-dissociation inhibitor 2,RhoGDI2)、肌球蛋白轻链2(myosin regulatory light chain 2,MLC2),它们与氧化还原平衡、信号转导通路、细胞骨架等功能有关。结论鉴定的差异表达蛋白可能与IPA的发病机制有关。

This study aimed to analyze differentially expressed proteins in the lung tissues of mice with invasive pulmonary aspergillosis （IPA） and to investigate molecular mechanism in the generation and development of invasive pulmonary aspergillosis at the level of proteomics. Two-dimensional eleetrophoresis （2-DE） was used to separate total proteins extracted from mouse lung tissues. Peptide mass fingerprintings （PMFs） were obtained, and then some spots on 2-DE gels were identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry （MALDI-TOF/YOF） combined with bioinformatics~ 4 spots were preliminarily identified as peroxiredoxin-6 （Prx6）, flavin reductase （FR）, rho GDP-dissociation inhibitor 2 （RhoGDI2）, and myosin regulatory light chain 2 （MLC2）, which were reported to involve in many biological processes including oxidation-reduction equilibrium, signaling transduction pathway and cell skeletal structure. From all result, we presumed that these differentially expressed proteins are probably related to pathogenesis of IPA.