β-arrestin1在实验性自身免疫性葡萄膜炎中的表达

Study on the expression of β-arrestin1 in experimental autoimmune uveitis

目的:初步探讨β-arrestin1在实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)中的表达。方法:成功建立EAU动物模型后,通过免疫组织化学和RT-PCR的方法检测β-arrestin1在EAU小鼠脾脏细胞中的表达。结果:免疫组织化学和RT-PCR均提示β-arrestin1在EAU小鼠脾脏细胞中的表达较正常小鼠增高。结论:β-arrestin1可能在EAU的发生发展中起着重要作用。

AIM：To study the expression of β-arrestin1 in the spleen cells of experimental autoimmune uveitis （EAU）. METHODS： The mice were injected into the foot with IRBP R16. On day 3, the spleen was extracted and the single celI suspension was made. Immunohistochemistry and RT-PCR were done on these spleen cells. RESULTS： The expression of β-arrestin1 on the spleen cells of EAU groups was much higher than that in the normal groups. CONCLUSION： β-arrestin1 might play an important role on the EAU.