手术创伤对成年鼠和老年鼠海马区GFAP、S100β表达的影响

Expression of GFAP and S100β in the hippocampus triggered by surgical trauma increases in aged rats

目的探讨肝部分切除术对成年鼠和老年鼠海马区胶质纤维酸性蛋白(GFAP)、S100β表达的影响。方法雄性成年SD大鼠和老年SD大鼠分别随机分为老年对照组、麻醉组和手术组以及成年对照组、麻醉组和手术组。手术组行肝部分切除术。实验鼠在术后1、3、7d行Morris水迷宫后,处死实验鼠取海马行免疫组化检测。结果 GFAP在成年鼠和老年鼠基础水平有统计学差异(P=0.039,P=0.002)。当增加的GFAP阳性细胞在成年鼠术后第3d(P=0.09)恢复后,老年鼠GFAP阳性细胞在术后第3d(P<0.001)仍然明显增加,在术后第7d(P=0.823)恢复。S100β与GFAP变化趋势相同。结论手术创伤引起成年鼠和老年鼠海马区GFAP、S100β可逆性表达增多,与成年鼠相比,老年鼠GFAP、S100β表达上调更加明显,持续时间更长。

Objective To investigate the effects of partial hepatectomy on spatial learning and memory and hippocampal GFAP and S100β in adult and aged rats. Methods Both adult and aged male SpragueDawley rats were randomly divided into 6 groups： control, anesthesia alone, and operation. The operation group underwent partial hepateetomy under general anesthesia. Rats were sacrificed on postoperative days 1, 3, and 7 after spatial working tests; perfused transcardially and postfixed for immunohistochemistry. Results Analysis of GFAP and S100β revealed significant effects of age （P〈0. 001, respectively） and operation （P〈0. 001, respectively）, and age× operation interaction （P〈0. 001, respectively）. Significant differences were observed for basal hippocampal GFAP and S100β between adult and aged rats （P=0. 039, P=0. 002, respectively）. While the GFAP expression in the adult subjects downregulated on day 3 （P= 0.09）, it remained significantly upregulated in aged subjects on day 3 （P〈0. 001） and improved on day 7 postoperatively （P=0. 823）. A similar pattern of change was seen with S100βConclusion The findings of this study indicate that moderate surgical trauma induces an elevated expression of GFAP and S100β in aged rats when compared to that of their adult counterrparts.