依达拉奉预处理对小鼠脑缺血再灌注损伤后皮质一氧化氮合酶表达的影响

Effect of Edaravone pretreatment on the expression of nitric oxide synthase in cortex of mice after cerebral ischemia reperfusion injure

目的探讨依达拉奉预处理对小鼠脑缺血再灌注(IR)损伤后皮质一氧化氮合酶(NOS)表达的影响。方法 48只健康ICR小鼠被分为假手术组、对照组和依达拉奉组。依达拉奉组和对照组分别给予依达拉奉3 mg/(kg.d)和同等体积的生理盐水腹腔注射共7 d,然后建立小鼠IR模型;缺血1 h、再灌注24 h时应用2,3,5-氯化三苯基四氮唑(TTC)染色法测量各组脑梗死体积,应用免疫组化法检测各组小鼠皮质神经元型、、诱导型和内皮型NOS(nNOS、iNOS、eNOS)阳性细胞数。结果与假手术组比较,对照组小鼠皮质nNOS、iNOS和eNOS阳性细胞数明显增多(均P<0.05);与对照组比较,依达拉奉组脑梗死体积明显缩小,皮质nNOS和iNOS阳性细胞数明显减少,eNOS阳性细胞数明显增多(均P<0.05)。结论依达拉奉预处理可以影响IR小鼠皮质nNOS、iNOS和eNOS的表达,发挥神经保护作用。

Objective To explore the effect of Edaravone pretreatment on the expression of nitric oxide synthase（NOS） in cortex of mice after cerebral ischemia reperfusion（IR） injure.Methods Forty-eight male ICR mice were rendomly divided into sham-operation group,control group and Edaravone group.Edaravone 3 mg/（kg·d） and same volume of normal saline were intraperitoneally injected in Edaravone group and control group respectively for 7 d.Then,the IR models were established.After 1 h ischemia and 24 h reperfusion,the volume of infarct was measured by 2,3,5-chlorinated three phenyl four nitrogen（TTC） dyeing method.The positive cells of neuronal,inducible and endothelial NOS（nNOS,iNOS,eNOS） in cortex were detected by immunohistochemistry.Results Compared with the sham-operation group,the quantity of nNOS,iNOS and eNOS positive cells in cortex in control group were significantly increased（all P〈0.05）.Compared with the contral group,the infarct volume in Edaravone group was significantly smaller,the quantity of nNOS and iNOS positive cells in cortex were significantly decreased,and the quantity of eNOS positive cells was significantly increased（all P〈0.05）.Conclusion Edaravone pretreatment has the neuroprotection through to change the expression of nNOS,iNOS and eNOS in cortex of IR mice.