应用电化学测菌法检测变形链球菌的初步探讨

Preliminary study of the ability of the electrochemical method for Streptococcus mutans detection

目的:探讨应用电化学测菌法检测变形链球菌的可行性。方法:利用本课题组前期建立的电化学测菌法检测变形链球菌,记录检测参数,并将电化学测菌法的检测结果与细菌培养结果进行比较。结果:①微流芯片样本通道内层流形成时间为(3.17±0.16)min,从进样到获得稳定电阻抗数据的时间为(10.20±0.16)min。②当变形链球菌浓度为104～109cells/mL时,细菌浓度C的对数与系统阻抗值Z呈良好的线性关系(R2=0.98)。③与细菌培养的检测结果相比,电化学测菌法获得的细菌数多,差异有统计学意义(P<0.05)。④变形链球菌浓度为105cells/mL时,电化学测菌法检测值与细菌培养法检测值之间的线性函数关系式为:y=0.94x+0.32E4(R2=0.95),两种方法的检测值高度正相关(P=0.001<0.01)。结论:电化学测菌法能为变形链球菌的快速检测提供新的思路。

Objectine： To investigate the feasibility of the electrochemical method that established in previous study based on microfluidic chip and impedance measurement to detect Streptococcus mutans. Method：The electrochemical method was used to detect Streptococcus mutans. Recorded certain test parameters of the detection system, meanwhile, compared the detection results with culture method. Result： （1）In microfluidic chip, the laminar form time was 3.17±0.16 minutes. From sample injection to stable impedance data collection only used 10.16±0.16 minutes. （2）In the concentration range of 10^4 to 10^9 ceils mL^-1,a linear relationship was found between the impedance magnitude and the logarithmic value of the cell concentration （R2=0.98）. （3）Compared with culture method, the more numbers of Streptococcus mutans was detected, and the differences was statistically significant （P 〈0.05）. （4）When the concentration of Streptococcus mutans is 105 cells mL^-1, the linear function formula is y=0.94x＋0.32E4 （R^2=0.95） meanwhile highly positive correlation between two methods （P = 0.001〈0.01）. Conclusion： The electrochemical method that we established previously might also be a rapid method to detect Streptococcus mutans.