氢溴酸东莨菪碱口崩片对小鼠神经行为学的影响

Influence of scopolamine hydrobromide orally disintegrating tablets on neurological behaviors in mice

目的考察基于纳米球-微粒(NiMS)系统的氢溴酸东莨菪碱口崩片对小鼠神经行为学的影响,为临床用药提供依据。方法本研究3项试验均按以下方法分组:40只小鼠,雌雄各半,分别将雌雄小鼠按随机数表编号,各分为4组,后将雌雄各5只合为一组,每组10只(对于协调能力的考察时,从50只小鼠中挑选出具有爬杆能力的40只),分别给予空白片及氢溴酸东莨菪碱口崩片高(24 mg/kg)、中(15 mg/kg)、低(6 mg/kg)3个剂量。(1)各剂量组小鼠灌胃给药20 min后置于自发活动记录仪内,通过记录小鼠活动次数观察药物对小鼠自发活动的影响。(2)小鼠灌胃给药20 min后腹腔注射阈下戊巴比妥钠,观察各组小鼠睡眠情况,考察药物对戊巴比妥钠的协同作用。(3)小鼠灌胃给药20 min后,进行爬杆实验,考察药物对小鼠协调能力的影响。结果 (1)与空白对照组相比,各剂量氢溴酸东莨菪碱口崩片对小鼠自发活动的影响差异均无统计学意义(P>0.05)。(2)随氢溴酸东莨菪碱口崩片剂量的增加,睡眠小鼠数量增加,高剂量组与空白组相比差异有统计学意义(P<0.05)。(3)小鼠服用氢溴酸东莨菪碱口崩片后,爬杆时间缩短,雌性小鼠给药各剂量组与空白组比较差异均有统计学意义(P<0.01);雄性小鼠给药高、中剂量组与空白组比较差异有统计学意义(P<0.01,P<0.05),低剂量组与空白组比较差异无统计学意义(P>0.05)。结论灌胃给予氢溴酸东莨菪碱口崩片对小鼠自发活动无影响,对阈下戊巴比妥钠的协同作用随药物剂量增大而增强,氢溴酸东莨菪碱口崩片还可以降低小鼠协调能力。

Objective To study the effects of scopolamine hydrobromide orally disintegrating tablets（DTs） based nanospheres-particles（NiMS） system on the neurological behaviors in mice,so as to provide evidence for clinical administration. Methods The 3 tests in this study were all divided as following： twenty male and 20 female mice were each randomly divided into 4 groups,with 5 mice in each group.Then a female and a male group were merged into one group,so there were 4 groups,each containing 10 mice.The four groups were subjected to the following treatments： control,high dose（24 mg/kg）,moderate dose（15 mg/kg） and low dose DTs（6 mg/kg）.To study the effects of DTs on the balance capacity,we selected 40 mice with the ability to climb the bar from 50 mice.（1） The spontaneous activities of mice were observed and recorded 20 min after intragastric administration of DTs in each group.（2） Pentobarbital sodium was intraperitoneally injected in mice 20 min after intragastric administration of DTs to observe the sleep of mice,so as to evaluate the synergistic effect between the two drugs.（3） Mice were put on the top of a stainless bar 20 min after intragastric administration of DTs and the time spent by the mice from top to the bottom of bar was recorded to evaluate the impact of the medication on mouse balance capacity. Results（1） DTs at each dose had no significant influence on the spontaneous activity of mice compared with the controls（P〉0.05）.（2） The sleep time of mice increased with the dose of DTs,and there were significant difference between control group with high dose and moderate dose groups（P〈0.05）.（3） DTs shortened the time spent by mice from top to the bottom of the bar compared with the control group,with significant differences found in female mice taking three doses of DTs（P〈0.01） and in male mice taking high and moderate dose of DT（P〈0.01,P〈0.05）. Conclusion Intragastric administration of DTs has no influence on spontaneous activity of mice,and its synergistic effect with pentobarbital sodium increases with dose.DTs can also decrease the balancing capacity of mice.