半胱氨酰白三烯受体1拮抗剂孟鲁司特对缺血性损伤后神经元形态的作用

Effect of montelukast on morphological changes in neurons after ischemic injury

目的:观察半胱氨酰白三烯受体1拮抗剂孟鲁司特对原代神经元缺血性损伤后形态变化的作用。方法:在不同浓度孟鲁司特存在条件下,以缺氧缺糖(OGD)2 h和再灌(R)24 h(OGD/R)诱导大鼠原代神经元及皮层混合培养细胞的神经元损伤;以免疫染色法检测神经元数量、胞体大小、胞体总神经突起数量、一级神经突起数量、长度及其分支数量等指标,观察神经元形态的变化。结果:OGD/R可明显减少神经元的数量,显著改变神经元的形态。而孟鲁司特(0.0001～1μmol/L)能减轻单独神经元培养中OGD/R诱导的神经元数量减少,抑制一级神经突起分支数量增加;在混合培养中,孟鲁司特(0.0001～0.1μmol/L)增加OGD/R后一级神经突的长度,减少神经突起数量以及一级神经突起分支数量。结论:半胱氨酰白三烯受体1拮抗剂孟鲁司特对大鼠原代神经元缺血性损伤有保护作用。

Objective： To determine the effect of montelukast, a cysteinyl leukotriene receptor 1 antagonist, on morphological changes in rat neurons after ischemic injury. Methods ： The in vivo ischemia injury was induced by oxygen-glucose deprivation （OGD） for 2 h and reperfusion （R） for 24 h （OGD/ R） in rat neurons primary culture and mixed cortex culture. In the presence or absence of various concentrations of montelukast, neuron number, area of neuron, number of neuritis per neuron, branch number of primary neuritis and primary neurite length were determined for evaluating morphological changes in neurons. Results： OGD/R significantly reduced neuron number, and ahered neuron morphology. In cortical neuron cultures, montelukast （0. 0001 - 1 μmol/L） attenuated OGD/R-inducedreduction in neuron number, and inhibited OGD/R-induced increase in branch number of primary neuritis. In the mixed cultures, montelukast （0. 0001 -0. 1μmol/L） increased the primary neurite length, and reduced number of neuritis and branch number of primary neurite after OGD/R. Conclusion ： Montelukast has a protective effect on ischemic injury in neurons.