摘要
目的探讨慢性丙型肝炎患者细胞因子诱导的杀伤(CIK)细胞体外诱导培养过程中各细胞亚群的变化。方法外周血单个核细胞(PBMC)在体外应用混合细胞因子(rhIFN-γ、IL-2和α-CD3)诱导培养,分别于0、7、10、13、15d时收集培养细胞,应用流式细胞技术检测各细胞亚群的频率。结果动态分析发现培养过程中CD3+细胞频率迅速增加,至10d时到达平台期;在整个培养过程中,CD3+CD8+T细胞和CD3+CD56+细胞比例持续增加,表现出良好的扩增能力,且与患者基线ALT水平无关。结论慢性丙型肝炎患者CIK细胞在体外培养的第10天,CD3+细胞呈现高扩增,且CD3+CD8+T细胞和CD3+CD56+细胞扩增状态良好,此时回输治疗可能效果较好。
Objective To explore the changes of the immune cell subsets during the process of cytokine-induced killer （CIK） cell incubation from patients with chronic hepatitis C. Methods Peripheral blood mononuclear cells （PBMC） from patients with chronic hepatitis C were in vitro incubated supplementing with eytokine cocktail （rhIFN-T, IL-2 and a-CD3）. On days at 0, 7, 10, 13 and 15, the incubated cells were collected and flow cytometry was performed to determine the frequencies of immune cell subsets. Results The frequencies of CD3 ^＋ cells increased quickly and reached the top values on the 10th day. The frequencies of CD3 ^＋ CD8 ^＋ and CD3 ^＋ CD56 ^＋ cells kept increasing during the cultureing procedure both in the patients with immune tolerant and immune active phase, which appearing strong capacity of proliferation. Conclusion CIK cells from patients with chronic hepatitis C would be suitable for autologous transfusion after in vitro incubation on the 10th day with higher levels of proliferation of CD3^＋ and high cell proliferation states of CD3^＋ CD8^＋ and CD3^＋ CD56^＋ cells.
出处
《肝脏》
2012年第5期311-314,共4页
Chinese Hepatology
