摘要
通过研究不同类型培养基和培养温度、起始pH值等发酵条件对IL-15产量的影响,初步确认了摇瓶水平发酵各因素的最优条件。优化的发酵条件为发酵起始pH值7.0、培养温度37℃、乳糖诱导浓度1.5 g/L、菌体生长密度OD600达到1.0时加入乳糖、诱导时间4 h,此时IL-15产量达到最高水平,从而为IL-15批量生产奠定了良好的基础。
The expression level of IL- 15 was influenced by different medium and other fermentation parameters including culture temperature, initial pH value and so on. The optimal parameters of various factors were confirmed by flask - fermentation. The optimal fermentation parameters with lactose induction was pH 7.0 of culture medium,37℃ of culture temperature, 1.5 g/L of joining lactose final concentration when the recombinant strain growth density OD60o achieved 1.0 and induction maintained for 4 hours,the production of IL - 15 reached the highest level. This fermentation technology established good foundation for the IL - 15 mass production.
出处
《中国兽药杂志》
2012年第6期34-37,共4页
Chinese Journal of Veterinary Drug
基金
吉林化工学院科研基金(2012-046)
关键词
IL-15
摇瓶发酵
表达
优化
interleukin - 15
flask - fermentation
expression
optimization
