西达本胺对胃癌SGC-7901细胞增殖、凋亡及Notch1、Sirt1基因表达的影响研究

Influence of chidamide on proliferation,apoptosis with expression of Notch1 and Sirt1 in gastric carcinoma cell line SGC-7901

目的:通过研究组蛋白去乙酰化酶抑制剂西达本胺对Notch1、Sirt1基因表达的影响来探讨其对胃癌细胞株SGC-7901的体外抗增殖作用。方法:采用MTT法观察不同浓度西达本胺对SGC-7901细胞增殖抑制作用,流式细胞仪(FCM)检测细胞凋亡率,RT-PCR检测Sirt1基因表达情况,蛋白质印迹法检测Notch1蛋白表达情况。结果:西达本胺在体外可抑制胃癌细胞SGC-7901增殖,呈浓度、时间依赖性;其可促进细胞凋亡,减少Sirt1基因的表达,下调Notch1蛋白的表达。结论:西达本胺可通过抑制Sirt1基因表达、下调Notch1蛋白的表达来抑制细胞增殖、促进细胞凋亡而发挥体外抗胃癌作用。

Objective： To investigate the influence of chidamide, a histone deacetylase inhibitor（HDACi）, on the proliferation inhibition and apoptosis effects to the human gastric carcinoma cell lines SGC-7901 and to explore the related mechanism of Notchl, Sirtl. Methods： SGC-7901 cells were incubated with chidamide at various concentrations and times. Cell growth inhibition was assessed by MTY. Cell apoptosis was analyzed by flow cytomerty（FCM）. The expression of Sirtl mRNA were determined by RT-PCR, the protein levels of Notchl were determined by Western blotting. Results： The proliferation of SGC-7901 cell was significantly inhibited and the apoptosis of SGC-7901 cell was promoted by chidamide（P 〈0.05）. The expression of Sirtl mRNA and the protein levels of Notchl were reduced by chidamide. Conclusion： Chidamide can promote apoptosis and inhibit proliferation by down-regulation of Sirtl and Notchl expression in gastric carcinoma cells.