1株吡嘧磺隆降解菌DF12的筛选和鉴定及降解特性

Isolation,identification and characterization of a Pyrazosulfuronethyl-degrading strain DF12

从湖南瑞泽农药厂污水池中筛选到1株吡嘧磺隆降解菌DF12,经形态学、生理生化及16S rDNA鉴定为恶臭假单胞菌(Pseudomonas putida),该菌在100 mL三角瓶中摇瓶体积70 mL、接种量2.0%(OD600=0.80)、pH7.0条件下对吡嘧磺隆降解率可达74.78%。

A Pyrazosulfuronethyl-degrading strain DF 12 was isolated from sewage reservoir of the herbicides factory in Hunan, which belonged to Pseudomonas putida based on physiological and biochemical properties and 16S rDNA analysis. When strain DF12 grown in LB medium was adjusted to an 00600 of 0.80 and inoculated in an inoculum size of 2.0% in 70 mL culture containing Pyrazosulfiaronethyl in a 100 mL flask with shaking under pH of 7.0, it could degrade 74.78 % of Pyrazosulfuron-ethyl.