长沙9个犬细小病毒毒株的分离与鉴定

Isolation and identification of nine canine parvovirus CS strains

从感染犬细小病毒(CPV)的长沙病犬粪便中分离CPV,采用同步接毒方式,将病毒悬液接种到猫肾细胞(F81),经PCR鉴定,从10份阳性样品中分离到9株CPV,血凝试验显示9株CPV均能凝集猪红细胞,血凝价均超过了27。为进一步分析CPV长沙毒株的VP2分子生物学特征及抗原变异规律,对9个CPV毒株的VP2基因进行克隆、测序及序列分析,结果表明:CPV长沙毒株与全国及世界各地CPV毒株相比,其VP2基因序列同源性超过97%,其VP2基因推导的氨基酸序列同源性超过94%;其VP2基因推导的氨基酸序列有独特的变异,且有独特变异的毒株在基因进化树上处于同一个分支。

Nine strains of canine parvovirus （CPV） were isolated using F81 cell from CPV positive feces collected from sick dogs in Changsha （CS）, the CPV strains were confirmed by PCR and hemagglutination assay showed all the virus isolates could agglutinate red blood cell of pigs with the titers all above 27. To further identify molecular and biological characteristics and the antigen variation of these isolates, VP2 gene was cloned, sequenced and analyzed. The results showed that the identities of gene sequence and the deduced amino acid sequence of CPV between the isolated strains and other reference strains were above 97% and 94%, respectively. And there were specific amino acid mutations in the VP2 of canine parvovirus CS strains.