对羟基苯甲酸酯类印迹微球的制备及条件优化

Synthesis and optimization of molecularly imprinted microspheres for ethyl paraben

以对羟基苯甲酸乙酯为模板,α-甲基丙烯酸为功能单体,甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁腈为引发剂,用沉淀聚合法制备对羟基苯甲酸乙酯分子印迹聚合微球。通过一系列的单因素实验优化了聚合反应条件,通过正交实验得出,在模板分子、功能单体、交联剂物质的量比例为1∶6∶20,反应温度为65℃的条件下合成的印迹聚合物的吸附量最大。以紫外-可见分光光度计对印迹聚合物的吸附性、选择性进行了分析。以优化后的反应条件合成的印迹聚合物对对羟基苯甲酸乙酯的相对吸附量为866.55μg/g,同等条件下非印迹聚合物的结果为406.72μg/g。

A novle molecularly imprinted polymer with specif- ic recognition ability for ethyl-paraben （EP） was prepared by precipitation polymerization,using methaerylic acid（MAA） as the functional monomer, ethylene dimethacrylate （EDMA） asthe linking agent,chloroform as porogenic solvents and azobi- sisobutyronitrile （AIBN） as the thermal polymerization initia- tor. The optimization condition was obtained by orthogonal ex- periments, the ratio of template molecule, functional mono- mer, crosslinker was 1：6：20, the reaction temperature is 65℃. The maximum adsorption was gotten under the optimi-zation conditions. The adsorbability and selectivity of imprin- ted polymer microspheres were measured by UV spectropho- tometer. The absorption of ethyl paraben by imprinted polymer microspheres is 866. 55μg/g, while the absorption by non- imprinted polymer microspheres is 406. 72 μg/g.