滇牡丹内生真菌PR35的鉴定及次生代谢产物的研究

Identification and secondary metabolites of endophytic fungal strain PR35 from Paeonia delavayi

目的:对滇牡丹内生真菌PR35菌株进行菌种鉴定,并对其次生代谢产物的化学成分进行研究。方法:采用形态学观察结合ITS rDNA序列分析法进行菌种鉴定;应用多种柱色谱方法对其次生代谢产物进行分离纯化,并依据其理化性质及波谱数据鉴定化合物结构。结果:菌株PR35鉴定为长梗木霉Trichoderma longibrachiatum;从菌株PR35的发酵产物中分离获得5个化合物,分别鉴定为1-(2,6-dihydroxyphenyl)-3-hydroxybutan-1-one(1),1-(2,6-dihydroxyphenyl)propan-1-one(2),1-(2,4,6-trihydroxyphenyl)butan-1-one(3),4-methoxy-1-naphthol(4)和啤酒甾醇(5),其中化合物1～3对灰葡萄孢、燕麦镰孢和紧密单孢枝霉显示明显的抗真菌活性。结论:内生真菌长梗木霉首次从滇牡丹植物中分离获得,5个化合物均为首次从滇牡丹内生真菌中分离得到,其中化合物4为首次从微生物发酵产物中获得。

Objective： To identify the endophytic fungal strain PR35 separated from Paeonia delavayi and study chemical con- stituents of its secondary metabolites. Method： The fungal strain PR35 was identified by morphological observation and ITS rDNA se- quence analysis. Various chromatographic methods were adopted to separate and purify its secondary metabolites, and their structures were identified by physiochemical properties and spectral data. Result： The fungal strain PR35 was identified as Trichoderma longibra- chiatum. Five compounds were separated from fermentation products of fungal strain PR35 and identified as 1-（2,6-dihydroxypheny1）- 3-hydroxybutan-1 -one （ 1 ）, 1 - （ 2,6-dihydroxypheny ） propan- 1 -one （ 2 ）, 1 - （ 2,4,6 -trihydroxypheny1 ） butan- 1 -one （ 3 ）, 4-methoxy-1 -naph- thol （4）, and cerevisterol （5）. Among them, compounds 1-3 showed notable antifungal activities against Botrytis cinerea, Fusarium aven- aceum and Hormodendrum compactum. Conclusion： The endophytic fungus T. longibrachiatum was separated from the plant P. dela- vayi for the first time. Five compounds were first separated from endophytic fungus of P. delavayi. Among them, compound 4 was sepa- rated from microbial fermentation products for the first time.