宁心红杞胶囊对去势雌性大鼠的雌激素样作用

Estrogen-like effects of Menoprogen on female ovariectomized rats

目的:研究宁心红杞胶囊对去势雌性大鼠的雌激素样作用机制。方法:采用去势雌性大鼠模型(OVX),以雌二醇(17β-estradiol,E2)作为阳性对照,给予宁心红杞胶囊2周后,测定其子宫系数、血清E2含量;以免疫组化法观察子宫上血管内皮生长因子(vascular endothelial growth factor,VEGF)、水通道蛋白(aquaporin,AQP)、雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)和原癌基因c-jun,c-fos的表达;以酵母双杂交法检测宁心红杞胶囊中是否含有与ERα或ERβ结合的成分。结果:宁心红杞胶囊与E2均能显著增加OVX大鼠子宫系数,提高血清E2水平,上调子宫上VEGF,AQP2和AQP5的表达;但与E2不同,未见宁心红杞胶囊有促进OVX大鼠子宫上ERα,PR,c-jun,c-fos表达的作用。酵母双杂交实验未检测到宁心红杞胶囊中含有与ERα或ERβ结合的成分。结论:宁心红杞胶囊具有雌激素样作用,能治疗围绝经期综合征,但作用机制不同于雌激素类物质,较少发生雌激素介导的子宫内膜癌的风险。

Objective： To study the estrogen-like action mechanism of Menoprogen on ovariectomized female rats. Method： Ovarieetomized rat model （OVX） was established and estradiol （17β-estradiol, E2 ） was used as positive control. The uterine coeffi- cient and serum E2 level were determined after administration of Menoprogen for 2 weeks. The uterine vascular endothelial growth factor （VEGF）, water channel protein （aquaporin, AQP）, estrogen receptor （ER）, progesterone receptor （PR） and the expression of pro- to-oneogenes（ c-jun, c-fos） were observed by immunohistochemieal method. Yeast two-hybrid assay was applied to detect the existence of components combining with ERα or ERβ in Menoprogen. Result： Both Menoprogen and E2 could significantly elevate the uterine co- efficient of OVX rats, increase the level of serum E2 and up-regulate the expressions of VEGF, AQP2 as well as AQP5 in uterus. E2, not as E2 Menoprogen couldn＇t promote the expressions of ERα, PR, c-jun and c-fos in OVX rat uterus. And yeast two-hybrid assay showed no components combining with ERα or ERβ in Menoprogen. Conclusion： Menoprogen has estrogen-like effect, and can be used to treat menopause syndrome. The risk of estrogen-mediated endometrial cancer is low for this treatment because its mechanism is different from estrogen-like substances.