丹参对糖尿病大鼠牙槽骨骨代谢及骨量变化影响的实验研究

Experimental study on effect of Salvia miltiorrhiza on alveolar bone metabolism and variation in bone mass in diabetic rats

目的:探讨丹参对糖尿病大鼠牙槽骨骨代谢及骨量变化的影响,以研究其对糖尿病导致的牙槽骨骨质疏松是否有抑制作用。方法:腹腔注射四氧嘧啶促使糖尿病大鼠形成,待血糖稳定并观察1周后应用丹参进行治疗观察。8周后所有大鼠禁食12 h后将大鼠处死,即刻空腹采血进行血糖及骨代谢指标测定。同时取大鼠的下颌牙槽骨固定进行骨密度(BMD)测定,并制作硬组织切片进行组织形态学观察。结果:糖尿病大鼠骨代谢指标出现不同程度异常,骨密度显著降低,经丹参治疗后症状有所缓解,各项指标得到改善,骨密度相应增高。结论:丹参对糖尿病大鼠早期的牙槽骨骨质疏松有一定的抑制作用。

Objective： To study the effect of Salvia mihiorrhiza on alveolar bone metabolism and variation in bone mass in dia- betic rats, in order to detect whether it has an inhibitory effect on alveolar bone osteoporosis caused by diabetics. Method ： Intraperito- Meal injection of alloxan induced diabetes in rats. After one week of observation and maintenance of stable blood sugar level, they were treated with S. miltiorrhiza. The rats were sacrificed at the eighth week after fasting for 12 h and blood samples were collected for anal- ysis of blood glucose and rate of bone metabolism. Meanwhile, their alveolar bones were collected for determining bone mineral density （BMD） and histological sections were made for histomorphology observation. Result： Diabetic rats showed varying degrees of abnor- mality in bone metabolism indicators and significant reduction in bone mineral density. After treatment with S. miltiorrhiza, their symp- toms reduced to some extend and all indicators were improved especially bone density. Conclusion： S. mihiorrhiza has a certain inhib- itory effect on alveolar bone osteoporosis in diabetic rats in early stage.