巴戟天补肾阳作用的血清代谢组学研究

Metabonomic study of intervention effects of Morinda officinalis on ` Kidney-yang deficiency syndrome'

目的:运用代谢组学方法研究氢化可的松诱导的肾阳虚大鼠血清代谢组变化及巴戟天补肾阳作用的机制。方法:通过核磁共振氢谱(1H-NMR)技术分析氢化可的松及巴戟天作用下的大鼠血清代谢物谱。应用正交偏最小二乘判别分析法研究对照组和模型组之间的代谢物谱差异,并通过变量重要性投影(VIP)在血清中发现了12种潜在生物标志物。采用主成分分析法研究巴戟天对大鼠肾阳虚证的干预效果。通过比较潜在生物标志物的含量差异,探讨巴戟天补肾阳作用的机制。结果:氢化可的松诱导的肾阳虚大鼠机体能量代谢、氨基酸代谢和肠道菌群都受到不同程度的影响,甲基转移反应被抑制。巴戟天可增强阳虚机体甲基转移反应、改善能量代谢和逆转氨基酸代谢紊乱,但对肠道菌群无明显作用。结论:代谢组学研究能较全面地反应生物体的生理及代谢状态,为中医"证"的本质研究及药物药效评价提供了一种新方法。

Objective： To investigate the metabolic profile of hydrocortisone-induced ＂Kidney-yang deficiency syndrome＂in rats and the intervention effects of Morinda offwinalis. Method： Proton nuclear magnetic resonance（^1H-NMR） technique was used to ana- lyze the rat metabonome in serum. Orthogonal partial least squares discriminant analysis （OPLS-DA） were processed to analyze the metabonome difference between the control and hydrocortisone treated samples. Twelve potential biomarkers were selected, via the pa- rameter of variable importance in the projection（VIP）. Principal components analysis （PCA） was employed to process the data from the M. officinalis, treatment group and the intervention effects of M. officinalis, was investigated through the selected potential biomar- kers. Result： After hydrocortisone treatment, the energy metabolism, amino acids metabolism and gut microflora environment were se- riously disturbed and transmethylation was surpressed. M. officinalis could effectively alleviate the disturbance of energy and amino acids metabolism and enhance transmethylation, but could not modulate the gut microflora environment. Conclusion： The results ob- tained suggested that metabonomic studies could better reflect the whole status of metabolism in bio-systems, and could be treated as a potential powerful approach in pharmacological studies and investigation of the essence of ‘syndrome＇ in traditional Chinese medicine.