超声波协同双水相体系提取茴香茎中总黄酮及其抗氧化性研究

Aqueous two phase system and ultrasonic wave coextraction of total flavonoids from Foeniculum vulgare stems and study on its antioxidation efficacy

将超声波技术和双水相体系萃取技术综合应用于提取茴香茎中总黄酮。通过单因素试验和正交试验相结合,得出影响茴香茎中黄酮提取因素的主要次序为提取液浓度>提取时间>料液比。最佳提取工艺条件为乙醇体积分数50%、料液比1∶15、超声时间20 min。抗氧化能力检测表明,该提取物抗氧化性显著,且浓度越高,其抗氧化性越强。

Uhrasonic extraction technology integrated with ethanol and ammonium sulfate aqueous two-phase separation was used to extracl total flavnnoids from Foen,iculum vulgare stem. Based on single factor and orthogonal experiment, the optimum extracting conditions were as follows： 50% of ethanol, solid to liquid ratio of 1：15 and ultrasonic extracting for 20 rain. The order of factors to affect total flawmes extraction was： ethanol concentration〉ultrasonic time〉solid to liquid ratio. Determination showed thai ifs antioxidation efficacv was enhanced with the increasing of its concentration.