硫脲壳聚糖铜配合物与牛血清白蛋白的相互作用

Interaction of thiourea-chitosan-copper complex with bovine serum albumin

在模拟动物体生理pH条件下,采用紫外吸收和荧光光谱研究硫脲壳聚糖铜(TUCS-Cu)配合物与牛血清白蛋白(BSA)的结合反应,探讨TUCS-Cu对BSA荧光猝灭过程的猝灭机理。结果表明,TUCS-Cu和BSA结合形成复合物,导致BSA内源性荧光猝灭机理属于静态猝灭。在室温下,TUCS-Cu与BSA的结合常数KA为2.68×106 L/mol。

In physiological condition, the binding reaction of thiourea-chitosan-copper （TUCS-Cu） with bovine serum albumin t BSA） was studied by uhraviolet spectrum and fluorescence spectrum. The results showed that TUCS-Cu combined with BSA to form a new comprmnd system, the quenching belonged to static fluorescence quenching. The binding constant （ K A） of TUCS-Cu with BSA at room temperature was 2.68×10^6 1· mol^-1.