吲哚美辛对结肠癌细胞HCT116的影响及机制探讨

Effects of indomethacin on the proliferation and invasion of colon cancer cell line HCT116 and its mechanisms

目的:研究吲哚美辛对结肠癌细胞株HCT116增殖及体外侵袭的影响并对其机制进行探讨。方法:应用WST-8法和体外侵袭小室测定吲哚美辛对结肠癌细胞株HCT116增殖和侵袭能力的影响;Westen-blotting方法检测吲哚美辛作用后HCT116细胞β-连环素(β-Catenin)、细胞周期素D1(Cyclin D1)、基质金属蛋白酶7(matrix metalloproteinase-7,MMP-7)蛋白的表达。结果:吲哚美辛能够明显抑制结肠癌细胞株HCT116的增殖,呈时间-剂量依赖性,作用24、48、72h的IC50值分别为440.36、323.90、254.37μmol/L;吲哚美辛能够明显抑制结肠癌细胞株HCT116的体外侵袭,呈剂量依赖性;吲哚美辛作用24、48、72h后,β-Catenin、CyclinD1、MMP-7蛋白表达均有下降。结论:吲哚美辛能抑制结肠癌细胞株HCT116的增殖与迁移,其作用机制之一可能是通过Wnt/β-catenin信号通路。

Objective To study the effects of indomethacin on the proliferation and invasion of colon cancer cell line HCT116 and its mechanisms. Methods The effects of indomethacin on the proliferation and invasion of colon cancer cell line HCT116 were measured by WST-8 method and Transwell chamber in vitro. The expressions of β-catenin, cyclin D1, and matrix metalloproteinase-7 （MMP-7） protein were detected by western blotting method after indomethacin treatment. Results The proliferation of HCT116 was inhibited by indomethacin in a time and dose dependent manner, and the IC50 of 24 h, 48 h, and 72 h were 440.36 μmol/L, 323.9 μmol/L, and 254.37 μβmol/L, respectively; and the invasion of HCT116 was also inhibited by indomethacin in a dose dependent manner. The expressions of β-catenin, cyclin D1, and MMP7 were reduced after indomethacin treatment, Conclusions Indomethacin can inhibit the proliferation and invasion of colon cancer cell line HCT116, which may through the Wnt/β-catenin signal pathway.