合并感染HIV对1b型HCV E2/NS1包膜区基因影响

Effects of HIV/HCV con-infection on E2/NS1 of HCV 1b genotype

目的研究人类免疫缺陷病毒(HIV)感染对1 b型丙型肝炎病毒(HCV)E2/NS1包膜区基因变异的影响,探讨2组间E2/NS1区基因序列同源性差异,为HCV/HIV合并感染者中HCV的治疗提供依据。方法对河南省某有偿献血村进行随访,将得到的所有HCV阳性病例255例根据合并HIV感染的情况分成2组,并对其基因分型,然后进行逆转录(RT)-巢式PCR扩增1 b型HCV E2/NS1包膜区基因,继而进行单链构象多态性分析(SSCP)、纯化后测序。结果 HCV单纯感染组和HIV/HCV合并感染组SSCP平均条带数分别为(3.4±0.55)、(2.6±0.55)条,差异有统计学意义(t=2.32,P=0.049);HCV单纯感染组和HIV/HCV合并感染组HCV E2/NS1包膜区同源性分别为76.7%和87.6%,差异有统计学意义(χ2=20.13,P<0.001);2组第一高变区(HVR-1)同源性分别为59.3%和81.9%,差异有统计学意义(χ2=10.39,P=0.001);2组第3高变区(HVR-3)同源性分别为71.7%和83.9%,差异有统计学意义(χ2=4.60,P=0.03);单纯HCV组中变异性较高的氨基酸位点在HCV/HIV合并感染组中表现出较高的保守性。结论 HIV感染对1b型HCV E2/NS1包膜区基因变异具有抑制作用,且其主要体现在HVR-1和HVR-3区。

Objective To study the change of E2/NS1 area of hepatctis C virus（HCV） caused by human immunodeficiency virus（HIV）/HCV co-infection and homologic differences between the two groups and to provide evidence for the treatment of HCV/HIV co-infection.Methods Totally 255 HCV positive cases were divided into two groups according to their HIV infection status and the HCV genotypes of the cases were identified.The E2/NS1 of HCV was amplified with reverse transcription-PCR（RT-PCR） for single strand conformation polymorphism（SSCP） analysis and sequenced after purification.Results There were statistical differences between the HCV/HIV co-infection group and the HCV mono-infection group in genotype of HCV（χ2=35.4,P0.001）.There were statistical differences between the HCV/HIV co-infection group and the HCV mono-infection group in SSCP strip number（t=2.32,P=0.049）.The homology of E2/NS1 of HCV for HCV mono-infection and HIV/HCV co-infection was 76.7% and 87.6%,respectively,with a significant difference（χ2=20.13,P0.001）.The variation in the two groups comes from HVR-1,HVR-2,and HVR-3.The homologic differences of the two groups are HCV E2/NS1,HVR-1,and HVR-3.The amino acid sites have high mutation in HCV mono-infection group but are more conservative in the HCV/HIV co-infection group.Conclusion HCV 1b is the main genotype in HCV mono-infection and HIV/HCV co-infection groups,but there are significant differences in HCV genotype distribution between the two groups.HIV can reduce the gene variant of 1b HCV E2/NS1,mainly in HVR-1 and HVR-3 area.