生物法高效合成氢过氧化亚油酸乙酯

Efficient Synthesis of Ethyl Linoleic Acid Hydroperoxide by Biotransformation

以亚油酸乙酯为底物,开发了一条利用豆粕为直接催化剂,在线偶联脂氧酶提取的高效合成氢过氧化物的方法。对影响氢过氧化亚油酸乙酯产率的各因素:温度、pH以及催化剂和底物用量等进行了研究。豆粕催化氧化亚油酸乙酯的最佳反应条件为:1.5 g亚油酸乙酯,1.5 g豆粕,0.45 mL乙醇溶于7.5 mL pH=6的磷酸盐缓冲液,反应温度15℃,反应时间2 h,在该条件下产率达55%。该法不仅省去提取、纯化等工序,而且提高了LOX反应的催化效率和产率,使生物转化过程更接近于工业化生产的需要。

With simultaneous enzyme releasing and lipoxygenation and using ethyl linoleate as substrate, the catalytic performance of soymeal in catalyzing the ＇hydroperoxidation reaction was evaluated. The influences of the various parameters affecting the yield of hydroperoxide, including pH, temperature, dosage of soymeal and ethyl linoleate, were investigated. The optimal conditions for ethyl linoleate were 1.5 g ethyl linoleate in 7. 5 mL phosphate buffer solution of pH 6 with 0. 45 mL ethanol as co-solvent reacting for 2 h at 15 ~C. Under these optimal conditions the yield reached 55% ,which is much higher than the previously reported figure（ 15% ）. Soymeal has much better catalytic performance than LOX solution. The application of soymeal not only reduces the number of steps by coupling extraction of LOX and oxidation reaction, but also increases the total yield.