北豆根不同组分发挥抗炎作用的安全范围研究

The Margin of Safety Study on Anti-inflammatory Effect of Different Components from Rhizoma Menispermi

目的研究北豆根水提、醇提组分发挥抗炎作用的治疗指数和安全范围。方法建立琼脂皮下注射致小鼠肉芽肿模型,给不同剂量的北豆根水提、醇提组分连续7天灌胃,观察北豆根不同组分抗炎作用,用Bliss法计算各组分发挥抗炎药效ED50、ED95;依据本实验室前文报道的北豆根不同组分急性毒性研究结果,用Bliss法计算各组分急性毒性的LD50,LD5,求得北豆根不同组分发挥抗炎作用的治疗指数(TI),安全系数(SF)。结果北豆根不同组分对炎症模型有明显的抗炎作用,呈现明显的量效关系,且醇提组分抗炎作用大于水提组分。北豆根水提、醇提组分对肉芽肿模型小鼠的抗炎安全范围TI、SF分别为57.532、17.4702和45.844、9.800。结论北豆根醇提组分对炎症模型的抗炎效果大于水提组分,但水提组分发挥抗炎作用的安全范围大于醇提组分。因此,在临床应用北豆根醇提组分时更应密切注意不良反应的发生并加强用药监测。

Objective To research therapeutic index（TI） and the margin of safety（SF） of and-inflammatory effect of Rhizoma Menispermi. Methods Establish granuloma model induced by subcutaneous injection of agar and administrate different doses of water extraction, alcohol extraction components of Rhizoma Menispermi to mice for seven days consecutively to observe anti-inflammatory effect of different components of Rhizoma Menispermi, using the Bliss method to calculate ED50 and ED95 of anti-inflarmnatory efficacy of the different components; According to earlier reports in our laboratory about the results of acute toxicity studies of different components of Rhizoma Menispermi, ED50 ED95, LD50 and LD5 are calculated by the Bliss method and the therapeutic index（TI）, safety factor（SP） of different com- ponents from Rhizoma Menispermi were assured by applying some formulas. Results The different components from Rhizoma Menispermi showed good granuloma effect, and showed certain dose-effect relationship, the anti-inflammatory effect of alcohol extraction componentis, is better than that of water extraction component. TI of the water extracts and the alcohol extracts from Rhizoma Menispermi is 57.532 and 17.4702, SF is 45.844 and 9.800 respectively. Conclusion The anti-inflammatory effect of the alcohol extracts is greater than the water extracts from Rhizoma Menispermi, but the margin of safety from water extracts is greater than the alcohol extracts from Rhizoma Menispermi. We should pay more attention to the occurrence of adverse reactions and strengthen drug monitoring in the application.