高效液相色谱法测定广西壮药毛郁金中姜黄素的含量

Determination of Curcumin in Rhizome of Curcuma aromatica by HPLC

采用Ultimate(XB-C18)色谱柱,以乙腈-4%冰乙酸(48∶52)为流动相,流速1.0 ml/min,检测波长430nm,柱温为室温,建立高效液相色谱法(HPLC)测定广西壮药材毛郁金中姜黄素含量的方法。结果表明,姜黄素在0.00992～0.08928μg范围内的峰面积与其浓度呈良好线性关系(r=0.9995),平均回收率为101.28%,RSD为1.67%(n=6)。本方法准确,快速,重现性好,可以作为广西壮药材毛郁金的含量测定方法。

A HPLC method was established to determine Curcumin in rhizome of Curcuma aromatica.Ultimate XB-C18 column was used as chromatographic column.The mobile phase was acetonitrile-4% acetic acid solution（48∶52）.The flow rate was 1.0 ml·min-1 and the detection wavelength was set at 430nm.The column temperature was maintained at room temperature.The calibration curve of Curcumin was in good linearity over the range of 0.00992～0.08928（μg）.The average recovery was 101.28%,with RSD of 1.67%（n=6）.This method is convenient,sensitive,reliable and suitable for determination of Curcumin in rhizome of Curcuma aromatica.