Zymobacter palmae菌株发酵甘露醇生产乙醇的条件优化

Condition Optimization of Ethanol Fermentation of Mannitol by Zymobacter palmae Strain

在250ml三角烧瓶中进行Zymobacter palmae菌株发酵不同浓度的甘露醇生产乙醇的实验。实验先以浓度为20.0g/L、40.0g/L、60.0g/L、80.0g/L的甘露醇进行发酵实验确定出最适培养基组分,然后通过正交试验确定出最佳发酵条件。结果表明,最适发酵培养基为:甘露醇20.0g/L,酵母膏0.5g/L,MgSO4.7H2O 0.1g/L,KH2PO42.0g/L,K2HPO47.0g/L,(NH4)2SO4 1.0g/L,pH值6.0;最佳发酵条件为:摇床转速150r/min,温度28℃,发酵液体积150ml。在最佳发酵条件下,乙醇的最大产量为0.429g乙醇/g甘露醇。

Mannitol was considered to be the main component in brown algae and used as research objects to investigate the condition optimization of ethanol fermentation with Zymobact palmae strain.The results showed that the optimal medium,in which mannitol was inverted to ethanol at high efficiency by Z.palmae in 250ml flask,was 20.0g/L mannitol,0.5g/L yeast extract,0.1g/L MgSO4·7H2O,2.0g/L KH2PO4,7.0g/L K2HPO4,1.0g/L（NH4）2SO4,pH value at 6.0.The best fermentation condition to produce ethanol was the rotation speed of 150r/min,temperature at 28 ℃ and fermentation liquor of 150ml,in which the highest yield reached to 0.429g ethanol/g mannitol.