微小RNA-21在弥漫大B细胞淋巴瘤中的表达及其临床意义

Significance of micro RNA-21 expression in diffuse large B-cell lymphoma

目的研究微小RNA-21（miR-21）在弥漫大B细胞淋巴瘤（DLBCL）中的表达，探讨miR-21表达与DLBCL发生、发展的意义及其与临床病理特征和预后的关系。方法采用实时荧光定量聚合酶链反应（RT—PCR）检测50例DLBCL存档蜡块和12例正常淋巴结中miR-21的表达，并采用免疫组织化学EnVision法检测bcl-2、p53在DLBCL中的表达。结果miR-21在DLBCL中高表达，且在非生发中心B细胞样（non—GCB）亚型中高于GCB亚型，50例DLBCL中有18例（36．0％）表达bcl-2，21例（42．0％）表达p53。DLBCL中miR-21表达水平与bcl-2呈负相关（P=0．020），与p53表达、DLBCL临床分期呈正相关（P=0．022；P=0．005），miR-21高表达的DLBCL患者3年生存率较miR-21低表达者低。结论miR-21高表达可能是DLBCL恶性度高的标志，是促进DLBCL肿瘤细胞增殖的重要因素，可能对预后有一定的指导意义。bcl-2、p53可能是miR-21在DLBcL中发挥作用的靶标。

Objective To investigate the expression of miR-21 in diffuse large B cell lymphoma （DLBCL） and normal lymph tissues and its potential relevance with clinicopathological characteristics. Methods The expression levels of miR-21 in 50 primary DLBCL and 12 normal lymph node tissue specimens were examined by TaqMan real-time polymerase chain reaction.The expression of bcl-2 and p53 was detected by immunohistochemistry staining. Results The expression of miR-21 was significantly higher in tumor tissues than that in normal tissues, in GCB subtypes higher than in non-GCB subtypes. And it was negatively correlated with bcl-2 （P=O.020）, while positively correlated with p53 （P=0.022）. Up-regulated miR-21 expression was low in three years of survival rate. Conclusion MiR-21 may indicate a more aggressive phenotype and serve as a molecular prognostic marker in DLBCL. High-expression of miR-21 is a key feature that is correlated with cell proliferation in DLBCL. miR-21 may have some guiding significance in prognosis, bcl-2, p53 is possibly one of the targets of miR-21 in DLBCL.