多发性骨髓瘤骨病患者白细胞介素-17的表达及其意义

Expression and significance of IL-17 in multiple myeloma bone disease

目的研究多发性骨髓瘤（MM）骨病患者骨髓中自细胞介素17（IL-17）的表达水平及其与骨髓单个核细胞核因子．（B受体活化因子配体（RANKL）表达的关系，探讨IL-17在MM骨病发病机制中的作用及其临床意义。方法采用双抗体夹心酶联免疫吸附（ELISA）法检测33例MM骨病患者及20例对照者骨髓上清中IL-17水平，采用荧光定量PCR检测上述两组骨髓单个核细胞RANKLmRNA的表达。结果MM骨病组及对照组的骨髓上清均表达IL-17，骨髓单个核细胞均表达RANKLmRNA。MM骨病组骨髓上清中IL-17的含量[（52．69±4．55）pg／ml]高于对照组[（14．35±1．25）Pg／m1]，MM骨病组骨髓单个核细胞RANKLmRNA的表达[（0．96±0．12）Pg／ml]高于对照组[（0．42±0．03）Pg／m1]，差异均有统计学意义（P〈0．05）。活动期MM骨病患者骨髓IL-17[（76．71±7．06）pg／m1]水平显著高于稳定期MM骨病患者[（40．67±3．84）Pg／ml]，差异有统计学意义（P〈0．05），活动期MM骨病患者骨髓单个核细胞RANKL mRNA的表达水平（1．22±0．27）显著高于稳定期MM骨病患者（0，83±O．12），差异有统计学意义（P〈0．05）。MM骨病组骨髓IL-17与RANKL的表达呈显著正相关（r=0．690，P〈0．05）。结论MM骨病患者骨髓IL-17的表达显著增高，骨髓IL-17水平与MM活动期和（或）稳定期相关，骨髓IL-17与RANKI．的表达呈正相关，I-17可能在MM骨病的发病机制中起重要的作用。

Objective To detect IL-17 level of bone marrow in patients with muhiple myeloma bone disease,and to investigate its clinical significance. Methods The bone marrow IL-17 levelswere quantified in 33 cases of multiple myeloma patients and 20 normal control by ELISA assay. RANKL mRNA expression were detected by using RT-PCR. Results In bone marrow supematant, IL-17 was detected in both groups, and RANKL mRNA were detected in both groups too. IL-17 and RANKL mRNA levels in bone marrow of patients with MM were significantly higher than those in the control group （P〈0.05）. The bone marrow concentrations of IL-17 and bone marrow mononuclear ceils＇ RANKL mRNA expression in active stage were significantly higher than those in stable stage （P〈0.05）. The bone marrow IL-17 and RANKL were significantly correlated （r = 690, P〈0.05）. Conclusion IL-17 may play an important role in the pathogenesis of MM.