EGF G61A多态性与食管鳞状细胞癌的关联研究

Epidermal growth factor（EGF） G61A polymorphism is associated with risk and clinicopathological characteristics of esophageal squamous cell carcinoma

目的 研究表皮生长因子基因（ ）G61A 多态性与食管鳞状细胞癌发生及其临床病理特征之间的关系.方法 采用聚合酶链反应- 限制性片段长度多态性（PCR-RFLP）方法检测366 例食管鳞状细胞癌患者（病例组）和400 例健康体检者（对照组） 61位点基因型和等位基因频率,分析两者组间分布及其与肿瘤临床特点之间的关系.同时,运用ELISA 法检测病例组和对照组血清EGF 水平.结果 61位点GG 基因型和G 等位基因频率在病例组和对照组中分布分别为61.2%、51.0%和79.2%、73.3%,差异均有统计学意义（P＜0.05）.GG 基因型患者易发生淋巴结转移（P＜0.05）.病例组血清EGF水平显著高于对照组,在两组内均以GG 基因型个体血清EGF水平高（P＜0.05）.结论 61 GG 基因型和G 等位基因可能与食管鳞状细胞癌相关,而且GG 基因型患者易发生淋巴结转移.

Objective To investigate the association of epidermal growth factor （EGF） G61A polymorphism with the risk and clinicopathological characteristics of esophageal squamous cell carcinoma. Methods EGF G61A polymorphism was de- tected by polymerase chain reaction - restriction fragment length polymorphism （PCR-RFLP） in 366 patients with esophageal squamous cell carcinoma and 400 health subjects. The serum EGF levels were measured and the pathological data were re- viewed. Statistical software SPSS 11.0（SPSS, Inc. ）was used data analysis. Results The distribution frequencies of GG genotype and G allele were 61.2%, 51.0% and 79.2%, 73.3% in patients and controls, respectively （P〈0.05）. Patients with genotype GG were more likely to have lymph nodes metastasis （P〈0.05）. The serum EGF levels of patients were higher than those of health subjects, and the serum EGF levels of the individual with genotype GG were higher than those with other genotypes （P〈0.05）. Conclusion EGF 61 GG genotype and G allele might be associated with risk of esophageal squamous cell carcinoma and GG genotype might be associated with lymph node metastasis in esophageal cancer.