酸水解对提高橘小实蝇蛋白饵剂中总游离糖含量的影响

Effects of acid hydrolysis on increasing the total free sugars in protein bait for Bactrocera dorsalis

为了提高蛋白饵剂中总游离糖的含量,采用酸水解的方法,通过单因素试验和正交试验对糖类水解酸浓度、水解温度和水解时间3个影响因素进行分析。在本试验范围内,最佳水解条件为酸(盐酸)浓度2.5mol/L,温度为95℃,时间为3h。水解酸(盐酸)浓度对糖类的水解影响最显著,其次是水解温度,水解时间影响最小。

The aim was to increase the total free sugars of protein baits. Acid hydrolysis was used to get free sugars from protein baits. The influences of acid concentration, the reaction temperature and the reaction time were analyzed using single factor experiment and orthogonal test. The results showed that the optimum conditions were 2.5 mol/L acid at 95 ℃ for 3 h. The hydrochloric acid concentration was the most important factor influencing the hydrolysis process, whereas the reaction time showed the slightest influence towards the hydrolysis process.