电针不同穴组对溃疡性结肠炎模型大鼠血清IL-6、IL-10含量的影响

Influence of electroacupuncture using difference combinations of points on serum levels of IL-6 and IL-1 in rat model of ulcerative colitis

目的:观察"俞募配穴"、"合募配穴"两种针刺配穴法治疗大肠腑病的效应差异,探讨"合治内府"之"合"的含义。方法:40只Wistar大鼠随机分为5组:空白组、模型组、曲池天枢组、天枢上巨虚组、天枢大肠俞组。酶联免疫吸附法(ELISA法)测定各组大鼠血清白介素6(IL-6)、白介素10(IL-10)含量的变化。结果:与空白组比较,模型组大鼠血清IL-6明显升高,IL-10明显降低,差异有统计学意义(P<0.01);模型组比较,各治疗组IL-6明显降低、IL-10明显升高,差异有统计学意义(P<0.01);与曲池天枢组比较,其它治疗组IL-6明显降低,IL-10明显升高,差异有统计学意义(P<0.05或<0.01);天枢大肠俞组与天枢上巨虚组比较,IL-6含量明显降低,差异有统计学意义(P<0.05)。结论:各治疗组对UC大鼠均有一定治疗作用,其中天枢配上巨虚组效果最佳,即下合穴配募穴治疗大肠腑病的效应优于俞募配穴及上合穴配募穴;"合治内府"之合穴应指"下合穴"。

Objective： To study the differential clinical effects of acupuncture using a combination of Back-Shu points and Front-Mu points and acupuncture using a combination of He points and Front-Mu points on large intestine diseases,and to discuss the meaning of ＂He＂ in ＂He therapy for internal organs＂.Methods： Forty Wistar rats were randomly divided into blank group,model group,Quchi/Tianshu point acupuncture group,Tianshu/Shangjuxu point acupuncture group,and Tianshu/Dachangshu point acupuncture group.Enzyme-linked immunosorbent assay（ELISA） was used to measure the serum levels of interleukin-6（IL-6） and interleukin-10（IL-10） in each group.Results： Compared with the blank group,the model group showed significantly increased serum IL-6 level and significantly decreased serum IL-10 level（P〈0.01）.Compared with the model group,all acupuncture groups showed significantly decreased serum IL-6 level and significantly increased serum IL-10 level（P〈0.01）.Compared with the Quchi/Tianshu point acupuncture group,other acupuncture groups showed significantly decreased serum IL-6 level and significantly increased serum IL-10 level（P〈0.05 or P〈0.01）.Compared with the Tianshu/Shangjuxu point acupuncture group,the Tianshu/Dachangshu point acupuncture group showed significantly decreased serum IL-6 level（P〈0.05）.Conclusion： Certain therapeutic effects on rats with ulcerative colitis have been shown in all acupuncture groups.The Tianshu/Shangjuxu point acupuncture group achieved the best therapeutic effect,that is to say,the acupuncture using a combination of Lower-He points and Front-Mu points can achieve a better therapeutic effect on large intestine diseases than acupuncture using a combination of Back-Shu points and Front-Mu points and acupuncture using a combination of Upper-He points and Front-Mu points.＂He＂ in ＂He therapy for internal organs＂ means Lower-He points.