血培养标本病原菌种类及耐药性分析

Study of pathogens detected from blood culture samples and its drug resistance

目的了解血培养阳性致病菌菌谱以及药敏情况,为抗生素合理使用提供理论依据。方法采用美国BD公司BACTEC9120型全自动血培养仪及其配套成人树脂需氧瓶和含溶血素厌氧瓶、儿童树脂需氧瓶培养,所有菌株按照常规操作流程进行鉴定。药敏实验采用K-B纸片扩散法,细菌菌谱及耐药性分析用WHONET 5.5软件。结果 1 290例血液培养标本中共分离出28种109株病原菌,阳性检出率为8.4%。革兰阳性球菌中未发现万古霉素耐药菌株。革兰阴性杆菌中,各菌属对抗菌药物均有一定的耐药率;均未发现亚胺培南和美罗培南耐药株。结论血液培养病原菌菌谱较广,以革兰阳性球菌为主,革兰阴性杆菌次之,且耐药情况普遍存在,应及时对血液细菌进行耐药性监测并指导临床合理使用抗生素。

Objective To investigate the distribution of pathogens and drug resistance in blood infection samples.Methods BACTEC9120 automated blood culture system and its corresponding adult aerobic resin bottles and bottles containing hemolysin anaerobic,aerobic resin flask children were used.All pathogens were identified in accordance with routine operating procedures.Drug sensitivity test was performed by K-B diffusion method and the results were analysed by WHONET 5.5 software.Results Totally 109 strains of 28 species pathogens were isolated from 1 290 blood culture samples,with the positive rate of 8.4%.Gram-positive cocci drugs tested were varied,but there were no vancomycin resistant strains found.Gram-negative bacilli was resistant to majority of drug tested but no imipenem（IPM） and meropenem（MEM）resistant strains were found.Conclusion Wide spectrum of pathogen is found in blood culture,and the primary pathogen is Gram-positive cocci,followed by gram-negativ bacilli.Drug resistance is prevalent,blood culture and drug resistance should be carried out in time to guide rational use of antibiotics.