茶碱与葛根素在大鼠体内药物动力学相互作用研究

Pharmacokinetic interaction studies of theophylline with puerarin in normal and hepatic fibrosis rats

目的研究葛根素对茶碱在大鼠体内药物动力学影响。方法 SD大鼠24只,分成四组:静脉给药对照组、静脉给药葛根素处理组、口服给药对照组和口服给药葛根素处理组,每组各6只,采用尾静脉注射或灌胃方式给予茶碱5 mg/kg,用HPLC法测定茶碱血药浓度,运用DAS 2.1.1拟合药动学参数,并采用SPSS 13.0软件进行统计分析。结果口服和静脉给药条件下,茶碱的药动学参数AUC0-24 h、AUC0-∞、CLz(或CLz/F)、MRT0-24 h、MRT0-∞和t1/2z在对照组和葛根素处理组之间差异均有高度统计学意义(均P<0.01),Cmax,Tmax,Vz(或Vz/F)在对照组和葛根素处理组之间差异无统计学意义(P>0.05)。结论茶碱在大鼠体内代谢受葛根素的影响显著。

Objective To investigate the pharmacokinetic interaction of theophylline and puerarin in rats.Methods Twenty-four Spraque-Dawley rats were divided into four groups（control group with intravenous injection,puerarin pretreated group with intravenous injection,control group with gavage administration and puerarin pretreated group with gavage administration） of which each got six rats and were administrated theophylline 5 mg/kg via gavage or tail intravenous injection.The concentration of theophylline in plasma was determined by HPLC method.The pharmacokinetic parameters were calculated by DAS 2.1.1 software and compared by SPSS 13.0 software.Results There was significant difference between control group and puerarin pretreated group for pharmacokinetic parameters of AUC0-24 h,AUC0-∞,CLz（or CLz/F）,MRT0-24 h,MRT0-∞ and t1/2z（P 0.01） under the condition of gavage and intravenous injection.There were no significant differences between control group and puerarin pretreated group for pharmacokinetic parameters of Cmax,Tmax,Vz（or Vz/F）（P 0.05）.Conclusion The metabolism of theophylline in rats is significant affected by puerarin.