人脑胶质瘤细胞中GDNF基因启动子1区甲基化状态的研究

Study of the Methylation Status of Promoter 1 Region of GDNF Gene in Human Glioma Cells

目的:观察GDNF启动子1区在人脑胶质瘤细胞中的甲基化修饰状态,以期探讨其对于GDNF在胶质瘤中高表达的影响。方法:基因测序检测10例胶质瘤与5例正常脑组织中GDNF基因序列,比较其基因是否有突变发生;重亚硫酸盐修饰后基因测序检测20例胶质瘤(10例低级别和10例高级别)与5例正常脑组织中GDNF启动子1区甲基化修饰状态。结果:GDNF启动子1区基因在胶质瘤中没有发生突变;GDNF启动子1区甲基化修饰在正常脑组织、低级别、高级别中发生率分别为72.25%、86.25%、86.75%。在胶质瘤中的甲基化修饰水平比正常脑组织明显增高(P<0.05),而高低级别之间无显著性差异。结论:在胶质瘤细胞中,GDNF启动子1区发生了高甲基化修饰,这种修饰很可能会影响GDNF基因的表达。

Objective： To observe the methylation status of GDNF promoter 1 region in human glioma,in order to explore the effect of methylation on the expression of GDNF in glioma.Methods： Genesequencing detected the GDNF gene order in 10 cases of glioma and 5 normal brain tissues,to compare whether their genetic mutations have occurred;the methylation modified status of GDNF promoter 1 region in 20 cases of glioma（10 cases of low-level and 10 cases of high-level） and 5 cases of normal brain tissues were detected through genesequencing after bisulfite modification.Results： The mutations of GDNF gene in promoter 1 region were not observed in glioma;the methylation of GDNF gene in promoter 1 region in normal brain tissues,low-level and high-level glioma were 72.25%,86.25%,86.75% respectively.The methylation level in glioma was significantly higher when compared to normal brain tissues（P〈0.05）;while there was no significant differences in high-and low-level tissues.Conclusions： Hypermethylation occuerred in GDNF promoter 1 region might influence the expression of GDNF in glioma cell.