复方甘草酸苷对溃疡性结肠炎大鼠结肠组织SOD、MDA、MPO酶的影响

Effect of Compound Glycyrrhizin on Activities of SOD、 MDA and MPO in TNBS-Induced Colitis Rats

目的探讨复方甘草酸苷对溃疡性结肠炎(UC)大鼠的治疗作用。方法将50只SD大鼠随机分成正常对照组(10只,不做任何处理)和模型组[40只,采用2,4,6-三硝基苯磺酸(TNBS)法复制UC大鼠模型]。造模3 d后,模型组又随机分成模型组对照组(0.9%氯化钠溶液)、复方甘草酸苷低、高剂量组(剂量分别为12.5,50 mg.kg-1.d-1)和阳性对照组(300 mg.kg-1.d-1美沙拉嗪溶液)。各组按设计剂量灌胃给药10 d后,取结肠组织观察病变程度并评分,并测定超氧化物歧化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)的含量。结果各治疗组肠炎病变评分和MDA含量与模型对照组比较显著下降(P<0.05),SOD、MPO未见统计学差异。结论复方甘草酸苷对UC的治疗作用可能与减轻或阻断组织的脂质过氧化反应及改善组织的病变程度有关。

Objective To study the protective effects of compound glycyrrhizin on inflammatory injury in ulcerative colitis rats. Methods Fifty rats were divided into normal control group （n = 10, untreated） and model group [ n = 40, colitis model rats were induced by 2,4,6-trinitrobenzene sulfonic acid （TNBS） ]. After 3 d, model group was divided into 4 groups： model control group （0.9% sodium chloride solution）, low-dose compound glycyrrhizin group （12.5 mg. kg-1 . d-1） , high-dose compound glycyrrhizin group （50 mg. kg-1. d-1 ）, and positive group （300 mg. kg-1. d-1 mesalazine solution）. According to each design dose to gavage after administration of 10 d, colonic mucosa was observed and histopathological scored, and the content of superoxide dismutase （SOD） ] malondialdehyde （MDA） and myeloperoxidase （MPO） was determined. Results In the treatment groups, the histopathological score and the content of MDA had significantly decreased compared with those of the model group （ P 〈 0.05 ）, whereas the activity of SOD and MPO had no significant changes. Conclusion Compound glycyrrhizin have protective effects on ulcerative colitis, which may be due to reduction or blocking of the lipid peroxidation of tissue and improvement of histopathological changes.