人二倍体化合子的DNA甲基化变化模式

DNA Methylation Patterns in Corrected Bipronuclear Human Zygotes

目的:探讨人类三原核合子及二倍体化合子中DNA甲基化模式的变化情况。方法:我们采用显微操作技术去除三原核合子中两个雄原核中的一个,观察恢复了二倍体状态的胚胎的发育情况,并检测了三原核和二倍体化的合子及早期胚胎中DNA甲基化模式的动态变化。结果:二倍体化的合子的囊胚形成率与三原核合子的囊胚形成率无显著性差异;在人三原核合子中两个雄原核发生主动地DNA去甲基化而雌原核在受精后的20h后仍保持甲基化。三原核与二倍体化合子中,DNA甲基化模式没有差别。结论:去除一个雄原核不会影响合子和胚胎的DNA甲基化模式。去除多余雄原核并不能改善胚胎的发育。

Objective： To investigate DNA Methylation Patterns in Corrected Bipronuclear（2PN） Human Zygotes.Methods： In this study,we removed one male pronucleus from tripronuclear zygotes.Then,development potentail of correct 2PN zygotes was compared with those of tripronuclear zygotes.We also examined the dynamic DNA methylation patterns in triploid and bipronucleated zygotes and early developing embryos.Results： We find that actively DNA demethylation of the two male pronuclei occurs in tripronuclear human zygotes while the female pronucleus remains methylated at 20 h post-insemination.Our data show that there are no differences in the DNA methylation between tripronuclear and corrected bipronuclear human zygotes.Conclusion： DNA methylation embryos and zygotes are not affected by removal of a male pronucleus.Diploidization does not improve embryo development potential.