细菌L型与胆囊结石的成因研究

Relationship between L-form bacteria and gallstones formation

目的 :探讨细菌L型在胆囊结石、胆囊炎发病中的意义。方法 :应用高渗培养技术对胆囊结石、胆囊炎病例 1 30份的胆囊胆汁标本进行了普通菌及L型菌联合培养。另外对其中胆囊切除标本行革兰染色 ,于光镜油镜下观察。结果 :胆囊疾病组细菌L型总检出率为 59 2 3 % ,对照组为 5 0 % ;各种类型胆囊结石胆汁中 ,以胆色素类结石检出率最高 ,为 88 89% ,其次为混合性胆石 (80 0 % ) ,胆固醇类胆石 (54 84% )和黑色结石 (50 0 % ) ,但各种类型的胆石之间无统计学意义上的差别。多发性结石明显高于单发性结石。 59例胆囊标本的革兰染色阳性率为83 1 % ,细菌L型感染受年龄因素影响 ,随年龄增加 ,其感染率增高。结论 :细菌L型感染在胆囊结石、胆囊炎发生发展中具有重要意义。临床上应重视细菌L型的致病性 。

Objective:To evaluate the role of L-form bacteria in gallstone formation.Methods:A L-form bacteriological study was made of 130 patients undergoing elective surgery for gallbladder diseases and 20 subjects with a normal biliary tract,as a control group,in addition,59 cases of gallbladder tissue slices were stained with Gram′s method.Results:The positive rate of bacterial L-form was 59 23% in benign gallbladder diseases and that of the control group was 5 0%.The positive rate of Gram′s staining in gallbladder tissue was 83 1%,According to percentage of L-form bacterial positive cultures,the pigment,mixted,cholesterol and black stones were 88 89%,80 00%,54 84% and 50 00% respectively.There was relationship between the number of stones in the gallbladder and the percentage of L-form bacterial positive cultures,as well as the age of patients.Conclusions:The results suggest that L-form bacterial infection play a certain role in stone formation in gallbladder,and the therapy for L-form bacterial infection in patents with gallstones and chronic cholecystitis should be applied in clinic.