白三烯A4水解酶基因多态性与结核性脑膜炎易感性的研究

Gene polymorphisms of LTA4H and susceptibility to tuberculous meningitis

目的探讨中国汉族人群中白三烯A4水解酶(LTA4H)基因多态性与结核性脑膜炎的相关性。方法应用SNaPshot技术检测48例结核性脑膜炎患者和70名健康对照者LTA4H基因中rs1978331,rs2247570,rs2660898,rs2660845多态位点的等位基因、基因型和单体型分布频率,采用SHEsis软件进行相关统计学分析。结果对照组与患者组rs1978331多态性位点C等位基因例数分别为83和40、频率分别为59.3%和41.7%,T等位基因例数分别为57和56、频率分别为40.7%和58.3%,差异有统计学意义(χ2=7.080,P=0.008);rs1978331位点CC、CT和TT基因型在对照组例和患者组中的例数分别为12、33、25和20、16、12,分布频率分别为17.1%、47.1%、35.7%和41.7%、33.3%、25.0%,差异有统计学意义(χ2=8.670,P=0.013);rs2660898位点AA、AC和CC基因型在对照组和患者组中的数分别为28、32、10和20、12、16,分布频率分别为40.0%、45.7%、14.3%和41.7%、25.0%、33.3%,差异有统计学意义(χ2=7.980,P=0.019);对照组与患者组单体型CACT频率分别为26.3%和45.8%,差异有统计学意义(χ2=7.349,P=0.007)。结论 LTA4H基因中rs1978331基因多态性位点等位基因、rs1978331和rs2660898基因多态性位点基因型、单倍体型CACT与中国汉族人群结核性脑膜炎的易感性存在相关性。

Objective To investigate the association between the gene polymorphisms of leukotriene A4 hydrolase（LTA4H）and tuberculous meningitis.Methods The genotypes and haplotype frequency of LTA4H rs1978331,rs2247570,rs2660898,rs2660845 alleles from 48 patients with tuberculous meningitis and 70 health controls were analyzed by SNaPshot.Differences in allelic and genotypic distributions between the case group and health control group were analyzed by the χ2 test,and were considered to be significant when P values were less than 0.05.All data were analyzed using SHEsis software.Results The following frequencies of 3 LTA4H alleles or genotypes between the controls and the cases had all significant differences： rs1978331 alleles between the controls（frequency of C alleles was 59.3% and of T alleles was 40.7%）and the cases（frequency of C alleles was 41.7% and of T alleles was 58.3%）（χ2=7.080,P=0.008）,rs1978331 genotypes between the controls（frequency of CC genotype was 17.1%,of CT genotype was 47.1% and of TT genotype was 35.7%） and the cases（frequency of CC genotype was 41.7%,of CT genotype was33.3% and of TT genotype was 25.0%）（χ2=8.670,P=0.013）,rs2660898 genotypes between controls（frequency of AA genotype was 40.0%,of AC genotype was 45.7% and of CC genotype was 14.3%） and cases（frequency of AA genotype was 41.7%,of AC genotype was25.0% and of CC genotype was 33.3%）（χ2=7.980,P=0.019）.The frequency of CACT haplotypes between controls（26.3%） and cases（45.8%） had also a significant difference（χ2=7.349,P=0.007）.Conclusion One allele（rs1978331）,two genotypes（rs1978331,rs2660898） and one haplotype（rs1978331,rs2247570,rs2660898,rs2660845： CACT）in LTA4H gene were correlated with tuberculous meningitis susceptibility in Chinese Han population.