期刊文献+

河南省结核分枝杆菌药物敏感性测试质量控制结果分析 被引量:4

Analysis of quality control results of drug susceptibility testing on mycobacterium tuberculosis in Henan province
下载PDF
导出
摘要 目的评价河南省Mtb药物敏感性测试的质量,规范并完善河南省结核病实验室药物敏感性室间质量保证体系。方法河南省6个受评估实验室按照《结核病诊断实验室检测规程》中比例法药敏试验要求对河南省结核病参比实验室下发的一线及二线药敏测试菌株58株进行比例法药敏试验,试验结果均报河南省结核病参比实验室(PRL),由PRL与标准结果比较并进行统计学分析。结果一线药物INH、RFP、S和EMB的一致性分别为91.0%(151/166)、95.8%(159/166)、89.2%(148/166)和77.1%(128/168);二线药物平均一致性达到了94.9%(683/720)。结论河南省6个评估实验室一线药的INH和RFP及四种二线药物敏感性检测均达到WHO和国家要求,EMB的敏感性检测水平有待进一步提高。 Objective To evaluate the quality control results of drug susceptibility testing(DST) of MTB in order to standardize and improve the external quality assurance(EQA)system of DST in Henan province.Methods In accordance with the TB diagnostic laboratory testing procedures,six laboratories intend to be evaluated performed the proportional method for DST proficiency testing.A total of 58 strains were issued by Henan provincial reference laboratory(PRL) to each testing laboratory,and the results were reported to PRL and made comparison with the standard results.Results The consistency of first line drug INH,RFP,S and EMB was 91.0%(151/166),95.8%(159/166),89.2%(148/166) and 77.1%(128/168) respectively.The average consistency of second-line drugs reached 94.9%(683/720).Conclusion The DST for first line INH and RFP and four second line drugs in six laboratories evaluated met the WHO and national requirement.However,the EMB testing should be further improved.
出处 《中国防痨杂志》 CAS 2012年第6期374-376,共3页 Chinese Journal of Antituberculosis
关键词 分枝杆菌 结核 微生物敏感性试验 质量控制 河南省 Mycobacterium tuberculosis Microbial sensitivity tests Quality control Henan province
  • 相关文献

参考文献7

二级参考文献12

  • 1柳正卫,何海波.浙江省第1轮结核分枝杆菌药敏试验室间质量保证结果分析[J].中国防痨杂志,2008,30(1):30-32. 被引量:5
  • 2杜长梅,王国斌,徐吉英,王国杰,胡惠义,甄新安,李爱华,孙占强,闫国蕊.河南省第二轮结核病耐药监测及耐药趋势研究[J].中国防痨杂志,2006,28(2):95-100. 被引量:26
  • 3WHO/IUATLD Global Working Group OnAntituberculosis Drug ResistanceSurveillance. Guidelines for surveillance of drug resistance in Tuberculosis. Geneva : WHO, 1996.
  • 4A. Laszlo, M. · Rahman, M. Espinal, etal. Quality assurance programme fordrug susceptibility testing of Mycobacterium tuberculosis in the WHO/IUATLD Supranational Reference Laboratory Network : five rounds of proficiency testing, 1994 - 1998 [ J ]. Int J TubercLung Dis,2002,6 (9) :748 - 756.
  • 5[3]A.Laszlo,M.Rahman,M.Espinal,et al.Quality assurance programme for drug susceptibility testing of Mycobacterium tuberculosis in the WHO/IUATLD Supranational Reference Laboratory Network:five rounds of proficiency testing,1994-1998[J].Int J Tuberc Lung Dis,2002,(6):748-756
  • 6[4]WHO/IUATLD Global Working Group on Antituberculosis Drug Resistance Surveillance.Guidelines for surveillance of drug resistance in tuberculosis.Geneva:WHO,1996
  • 7A. Laszlo, M. Rahman, M. Espinal, M. Raviglione. Quality assurance programme for drug susceptibility testing of Mycobacterittzn tuberculosis in the WHO/IUATLD Supranational Reference Laboratory Network: five rounds of proficiency testing, 1994--1998[J]. Int J Tuberc LungDis,2002,(6):748--756.
  • 8WHO/IUATLD Global Working Group on Antituberculosis Drug Resistance Surveillance. Guidelines for surveillance of drug resistance in tuberculosis[M]. Geneva: WHO, 1996.
  • 9中国防痨协会基础专业委员会.结核病诊断实验室检验规程.北京:中国教育出版社,2006,46-51.
  • 10缪梓萍,李群,何海波,柳正卫,杨石波,郑锦雷,刘北斗.浙江省耐药结核病现状及趋势研究[J].中国防痨杂志,2007,29(3):215-218. 被引量:49

共引文献138

同被引文献36

  • 1包洪,于庭,印璞,刘爱忠,张吉平.中国部分地区结核分支杆菌耐利福平、异烟肼和链酶素耐药基因的检测[J].中国实验诊断学,2007,11(2):232-234. 被引量:8
  • 2中华人民共和国卫生部.全国结核病耐药性基线调查报告(2007-2008年)[M].北京:人民卫生出版社,2010.25-52.
  • 3陈明亭,万康林.结核病实验室技术手册[M].北京:科学出版社,2011:34-68.
  • 4中国人民共和国卫生部. 全国结核病耐药性基线调查报告(2007-2008 年)[M]. 北京:人民卫生出版社,2010. 1-4.
  • 5中国防痨协会基础专业委员会. 结核病诊断实验室检测规程[M]. 北京:中国教育文化出版社,2006. 46-51.
  • 6Laszlo A, Rahman M, Espinal M, et a1. Qualityassurance programme for drug susceptibility testing ofMycobacterium tuberculosis in the WHO/IUATLD Supranational Reference Laboratory Network: five rounds ofproficiency testing, 1994-1998[J]. Int J Tuberc LungDis,2002,6(9):748-756.
  • 7World Health Organazation. Global tuberculosis report 2013 [ M ]. Geneva : World Health Organization,2013.
  • 8Hu Y, Hoffner S, Jiang W, et al. Extensive transmission of isoniazid resistant M. tuberculosis and its association with increased muhidrug-resistant TB in two rural counties of eastern China: a molecular epidemiological study[ J]. BMC Infect Dis, 2010, 10(43) :1-8.
  • 9[ Van Deun A, Wright A, Zignol M, et al. Drug susceptibility testing proficiency in the network of supranational tuberculosis reference laboratories[ J ]. Int J Tuberc Lung Dis, 2011, 15 (1) : 116-124.
  • 10Somoskovi A, Parsons LM, Salfinger M. The molecular basis of resistance to isoniazid, rifampin, and pyrazinamide in Mycobacterium tuberculosis[ J]. Respir Res, 2001, 2 (3) : 164- 168.

引证文献4

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部