不同地区HCV感染病人同一基因型(1b)膜区(E_2)序列比较和分析

Sequence Analysis of HCV E_2(HVR) Region of Same Genotype Obtained from Different Regions.

用采自中国河南省 (HN)、河北省 (HB)及瑞典 (SW )HCV感染者的血清标本 ,研究不同地区HCV感染病人同一基因型 (1b)膜区 (E2 )基因变化。利用RT—PCR对HCV膜区基因扩增并测定序列。结果显示不同地区分离的同一基因型HCV膜区序列各异 ,相同地域基因同源性较高。变化最大的区域是HVR区 ,核苷酸变化导致了氨基酸序列改变。

To study gene variation of HCV E 2 region (HVR) of same genotype isolated from patients with HCV infection collected from different geographical areas.Serum samples of patients with HCV infection were collected from Hebei,Henai provinces of China and Sweden.HCV E 2 region was amplified by RT-PCR with type-specific primers targeted on E 2 region,and then sequenced.The sequences of HCV E 2 gene isolated from different and then sequences.The sequences of HCV E 2 gene isolated from different geographical areas were not identical,with the highest variable region in HVR.The homology of the HCV E 2 sequences from patients in the same area was higher than that from different regions.The mutation of nucleotide caused changes of amino acid.Gene variation of HCV closely related to the geographical region.