苯乙酸诱导分化胶质瘤细胞过程中基因表达变化

The change of P16、C myc gene protein expression during differentiation induced by phenylacetate on glioma cell line C6

目的 观察诱导分化剂苯乙酸对胶质瘤细胞 C6的增殖分化过程中癌基因 C- myc,抑癌基因 P1 6的蛋白水平变化。方法 3H- Td R掺入法测细胞增殖率 ;免疫组化 SP法检测 C- myc、P1 6基因蛋白水平的表达。结果 应用苯乙酸后 ,细胞 CPM值降低 ;C- myc基因胞浆阳性表达下降 ,P1 6基因胞浆阳性表达升高。结论 苯乙酸对胶质瘤细胞存在剂量依赖性抑制 ,作用方式为抑制胞浆中 RNA合成。这些作用与苯乙酸降低了 C- myc基因对细胞恶性增殖的转录调节作用 ,增强了抑癌基因 P1 6活性有关。

To observe the changes of protein expression of C myc,P16 gene on glioma cell line C6 treated by phenylacetate(PA) in order to find out the mechanism of PA on molecule level.Methods The effect of PA on cell proliferation using 3 H TdR assay,C myc and P16 gene protein expression studied by immunohistochemical technique.Results At does ranges 0 625、2 5、5 0 mM,PA revealed a dose dependent proliferation inhibitory effect.The protein expression of C myc gene was notably decreased,while P16 gene was increased by PA(0 625&2 5 mM).Conclusion PA caused a does dependent proliferation inhibitory effect,one of the mechanisms may be PA decreased RNA amount directly in cells.All of these connected with C myc gene protein expression deceased and P16 gene activity increased by PA.