木霉制剂对海州香薷生长和铜吸收的影响

Effect of Trichoderma sp. preparation on plant growth and Cu accumulation of Elsholtzia splendens

将里氏木霉FS10-C用苜蓿粉发酵制成固体木霉制剂.采用温室盆栽试验,研究在外源铜浓度为0,100,200,400mg/kg的土壤上,施加木霉制剂对海州香薷生长和铜吸收的影响.结果表明:施加木霉制剂后,除400mg/kg外源铜浓度土壤上地上部干重外,海州香薷生物量在各外源铜浓度土壤上均呈增加趋势.施加木霉制剂的各外源铜浓度土壤NH4OAc提取态铜含量均显著高于空白组(P<0.05),且除100mg/kg外源铜土壤上地上部外,海州香薷铜积累量均呈增加趋势.其中地上部积累量在200和400mg/kg外源铜土壤上均差异显著(P<0.05),根积累量在100mg/kg外源铜土壤上差异显著(P<0.05),总积累量在100和400mg/kg外源铜土壤上均差异显著(P<0.05).可见,里氏木霉FS10-C制剂可通过促进植物生长和提高土壤铜的植物有效性而提高海州香薷对铜的吸收能力,是一种颇具研发潜力的生物修复剂.

Spores of Trichoderma reesei FS10-C were made into Trichoderma sp.preparation with alfalfa powder by solid-state fermentation.And a pot experiment was carried out to investigate effect of the preparation on plant growth and Cu accumulation of Elsholtzia splendens in soils spiked with 0,100,200 and 400 mg/kg of CuSO4.The results indicated that the plant biomass of Elsholtzia splendens showed an increasing trend in soils with all spiked Cu concentrations after applying of the preparation,except for shoot dry weight in soils with external Cu levels of 400 mg/kg.The preparation increased NH4OAc-extractable Cu in all Cu contaminated soils significantly in comparison with that in the control（P0.05）,and also led to an increasing trend in Cu accumulations of all plants,except for that in shoot in soils with external Cu level of 100 mg/kg.Cu accumulations were significantly enhanced in shoots in soils with external Cu levels of 200 and 400 mg/kg,in roots in soils with external Cu levels of 100 mg/kg and in individual plants in soils with external Cu levels of 100 and 400 mg/kg（P0.05）.The results suggested that this Trichoderma reesei FS10-C preparation could be a potential bioremediation agent for Cu contaminated soil by promoting growth of Elsholtzia splendens and increasing Cu phytoavailability in soil.