不同诱导培养基对小麦花药培养胚状体诱导率的影响

Effect of Different Media on the Induction Rate of Embryoid in Wheat Anther Culture

为明确W14-F诱导培养基对黑龙江省春小麦品种及人工合成小麦材料的花药培养胚状体诱导率,以6份人工合成小麦材料和4个普通小麦品种为材料,采用4种诱导培养基W14-F、MS、N6和C17进行花药培养比较试验。结果表明:不同的诱导培养基对于小麦材料的胚状体诱导率不同,4种诱导培养基的胚状体诱导能力依次为W14-F>C17>N6>MS,W14-F培养基胚状体诱导率明显高于其它3个培养基。人工合成小麦和普通小麦在4种培养基上都得到了相近的胚状体诱导率,说明W14-F培养基同样适合人工合成小麦。利用该技术体系培养人工合成小麦与普通小麦的杂种F1材料,得到了较高的诱导率,这为该技术体系直接应用于利用人工合成小麦改良小麦品种抗病抗逆性研究中提供了可靠的依据。

In order to clarify the effect of W14-F induction media on induction rate of embryoid of synthetic hex-aploid wheat and common wheat, 6 synthetic hexaploid wheat accessions and 4 common wheat varieties were cultivated on four induction media：Wl4-F, MS,N6 and C17. The results showed that different induction media got different induction rate of embryoid,W14-F〉Cl7〉N6〉MS,the induction rate of W14-F was higher than that of the other media evidently; The research on the comparison of synthetic hexaploid wheat and common wheat showed that there was no difference between synthetic hexaploid wheat and common wheat cultivars a- dapted in Heilongjiang on the rate of embryoid in anther culture. The F1 derived from crosses of synthetic hexa-ploid wheat and common wheat got a higher induction rate of embryoid by this technology system, indicating that W14-F medium could be important for enhancing efficiency of breeding for resistance to bio- and abio-stress using synthetic hexaploid wheat.