差热分析法和近红外漫反射光谱法快速鉴别不同栽培品种的苦荞麦

Identification of different breeds of Fagopyrum tataricum by differential thermal analysis and near infrared reflectance spectroscopy

目的用差热分析法(DTA)和近红外漫反射光谱(NIRS)法对11种栽培苦荞麦进行快速分析鉴别。方法应用TG-DTA热重差热联用仪分别对11个栽培品种苦荞麦进行差热图谱扫描与分析。实验条件为差热量程:±50μV;升温速率:15℃/min;升温范围:35℃～500℃;气氛为氮气,流速:50 ml/min。应用Bruker Vector 22/N型傅立叶变换近红外光谱仪对11种栽培苦荞麦进行扫描。实验条件为测定方式:积分球漫反射;扫描次数64次;扫描范围:3 700～12 000/cm。结果结合两种方法能快速鉴别"建德苦荞麦"同其他苦荞麦,鉴别效果良好。结论 "建德苦荞麦"作为一个新的培育品种,可以对其营养成分、药理活性进行进一步评价,为广泛推广提供参考。

Objective To identify different breeds of Fagopyrum tataricum by differential thermal analysis and near infrared reflectance spectroscopy. Methods TG/DTA and thermal analysis were both used to scan and analyze the thermal map of 11 breeds of F. tartaricum. The experiment conditions included DTA range of ± 50 uV, a calefactive rate of 15℃/min, an ambience of nitrogen and flow velocity of 50 ml/min. Bruker Vector 22/N-type Fourier transform near infrared spectroscopy was used to scan and analyze of 11 breeds F. tartaricum. Experiment conditions were： integrating sphere diffuse reflectance as measure methods; scan for 64 times; 3 700 - 12 000/cm as scan range. Results Both methods could identify the Jiande tartary buckwheat from other breeds. Conclusion Jiande tartary buckwheat was a new cultivar, nutrient value and pharmacological activity of which should be evaluated for promotion.