HPLC法测定葛花中鸢尾苷和鸢尾苷元的含量

Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

目的建立测定葛花中鸢尾苷和鸢尾苷元含量的高效液相色谱方法。方法采用美国Agilent-C18柱(4.6 mm×250 mm,5μm),以甲醇-乙腈-水(2∶1∶2)为流动相,流速为1.0 ml/min;检测波长为264 nm,柱温为室温。结果鸢尾苷在2.0～120.0μg/ml呈线性关系,r=0.999 8;平均加样回收率为99.7%,RSD为1.9%;鸢尾苷元在0.8～80.0μg/ml呈线性关系,r=0.998 6;平均加样回收率为99.4%,RSD为1.7%。结论该方法简便易行,结果准确、可靠,重复性好,可用于葛花中鸢尾苷和鸢尾苷元的含量测定。

Objective To establish an HPLC method for the determination of Tectoridin and Tectorigenin in Flos Puerariae lobata. Method The separation was performed on Agilent -C18 column（4.6 mm × 250 mm,5 um） ,using a mobile phase with methanolacetonitrile-water（2： 1： 2）. Flow rate was 1. 0ml/min,the detection wavelength was set at 264 nm. Column temperature was ambient temperature. Result The linear range of tectoridin was 2.0 - 120.0 ug/ml,r = 0. 999 8. The average recovery was 99.7% ,RSD was 1.9% ;The linear range of tectorigenin was 0.8 -80.0 ug/ml, r = 0. 998 6. The average recovery was 99.4% ,RSD was 1.7 %. Conclusion The method was simple ,accurate,reliable with a good repeatability,which could be used to determinate of tectoridin and tectorigenin in Flos Puerariae lobata.