中国七日热型、澳洲型钩端螺旋体分子流行病学调查

A molecular epidemiological investigation on Leptospira interrogans serovar hebdomadis and australia in China

目的 确定耕牛是否为七日热型、澳洲型钩端螺旋体病的传染源。方法 自钩端螺旋体病患者血液及耕牛尿中分离出的 12株七日热型、澳洲型钩端螺旋体野生株染色体DNA用限制性内切酶EcoRⅠ酶切后 ,进行染色体DNA限制性内切酶图谱及 16S +2 3SrRNA基因限制性内切酶片段长度多态性分析。结果 自钩端螺旋体病患者血液及耕牛尿中分离的同一血清型的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型相同。血清型不同的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型则不相同。结论 耕牛是我国七日热型及澳洲型钩端螺旋体病的传染源 。

Objective In order to confirm whether cattle serve the source of infection for patients with Leptospira interrogans serovar hebdomadis and australia. Methods 12 field strains of leptospira interrogans serovar hebdomadis and australia were isolated from blood samples of patients with leptospirosis and farm cattle urine. An analysis of chromosome DNA restriction endonuclease patterns (REP) and restriction fragments length polymorphism (RFLP) of 16S+23S rRNA gene were processed by digestion of chromosome DNA using EcoR I. Results The same serovar field strains of Leptospira interrogans from blood samples of patients with leptospirosis and farm cattle urine resulted in unique restriction endonuclease patterns (REP) and ribosomal types (RT).The chromosome DNAs from field strains that belonged to different serovars of Leptospira interrogans caused different restriction endonuclease patterns and ribosomal types. Conclusion In accordance with this results, we recognize that farm cattle serve the infectious source of leptospirosis serovar hebdomadis and australia thus may have realistic significance in the control of leptospirosis serovar epidemic of hebdomadis and australia in China.