枯草芽孢杆菌C3产抗菌物质发酵培养基的优化

Optimization of fermentation medium for antimicrobial substances from Bacillus subtilis C3

该文利用对黑曲霉孢子萌发有抑制作用的枯草芽孢杆菌C3,研究优化产抗菌物质的发酵培养基组分。在碳源、氮源、C/N单因素多水平试验结果的基础上,设计Na+、Mg2+、K+浓度3因素3水平L(933)正交试验,研究无机盐离子对枯草芽孢杆菌产抗菌物质的影响。结果表明,碳源为1%葡萄糖,氮源为蛋白胨和酵母浸粉各1.5%,碳氮比为1∶3,NaCl为0.5%,KH2PO4为0.1%,MgSO4.7H2O为0.15%时,枯草芽孢杆菌产抗菌物质的抑菌效果明显,对霉菌孢子萌发的抑制率提高了118.64%。

Using Aspergillus niger spore germination of an inhibition of Bacillus subtilis C3,this paper optimizes antifungal substances of fermentation components for research.Based on the test results of carbon source,nitrogen source,C/N single factor with many levels,we design three factors three levels L9（33） orthogonal test of Na＋,Mg2＋,K＋ concentration to test the influence of inorganic salt ion to Bacillus subtilis antibacterial material.The results show that when the carbon source is 1% glucose,nitrogen source is peptone and yeast extract 1.5% each,C/N is 1 ∶ 3,NaCl is 0.5%,KH2PO4 is 0.1%,MgSO4·7H2O is 0.15%,the antibacterial effect from Bacillus subtilis antibacterial substance is obvious and the inhibition rate of fungus spore germination increases 118.64%.